A new rat model for investigation of subglottic stenosis. (March 2013)

• Record Type: Journal Article
• Authors: Woo, Joo Hyun; Han, Gyu Cheol; Kang, Il Gyu; Kim, Sun Tae; Cha, Heung Eog; Kim, Dong Young
• Is Part Of: Acta oto-laryngologica. Volume 133:Number 3(2013)
• Page Start: 276
• Page End: 280
• Publication Date: 2013-03
• Subjects: Otolaryngology -- Periodicals; Ear -- Diseases -- Periodicals; Throat -- Diseases -- Periodicals; Otolaryngology -- Electronic Resources; Otorhinolaryngologic Diseases; 617.8
• DOI: 10.3109/00016489.2012.734930
• Languages: English
• ISSNs: 0001-6489

Abstract

<abstract> <title>Abstract</title> <p> <italic>Conclusion:</italic> This study attempted to develop a new rat model of subglottic stenosis (SGS), resulting from subglottic mucosal injury administered by electrocauterization. Despite failure of the posterior SGS model, the anterior SGS model was considered feasible. <italic>Objective:</italic> To investigate the feasibility of using rats as an animal model for SGS. <italic>Methods:</italic> Thirty-seven female Sprague-Dawley rats were assigned to the control group or to the anterior or posterior subglottic injury group. Electrical cauterization was performed on the anterior or posterior 180° of the subglottic mucosa. Animals were euthanized at 4 weeks after injury. Histologic features of the subglottis were evaluated as regards changes in the subglottic lumen, lamina propria, cartilage, and epithelium after hematoxylin and eosin and Masson trichrome staining. <italic>Results:</italic> Survival in the anterior injury group was 80% (13/15) until 4 weeks, and results of histologic evaluation showed an increase in thickness of lamina propria with fibrosis and cartilage damage, resulting in luminal narrowing. A high rate of mortality was observed in the posterior injury group.</p> </abstract>