Evaluation of the cytotoxicity of cigarette smoke total particulate matter using three in vitro assays and two types of cells. (May 2013)

• Record Type: Journal Article
• Authors: Li, Xiang; Peng, Bin; Nie, Cong; Shang, Pingping; Liu, Huimin
• Is Part Of: Toxicology mechanisms and methods. Volume 23:Number 4(2013)
• Page Start: 240
• Page End: 246
• Publication Date: 2013-05
• Subjects: Analytical toxicology -- Periodicals; Toxicology -- Periodicals; Toxicology -- Methodology -- Periodicals; 615.907
• DOI: 10.3109/15376516.2012.743640
• Languages: English
• ISSNs: 1537-6516

Abstract

<abstract> <title>Abstract</title> <p> <italic>In vitro</italic> cytotoxicity assays can be used to evaluate potential toxicological effects of tobacco products. Total particulate matter (TPM) from mainstream cigarette smoke trapped by a Cambridge filter is used widely for biological evaluation of smoke. This study compared neutral red uptake (NRU), lactate dehydrogenase (LDH) activity and WST-1 assays for assessing the cytotoxicity of TPM, and evaluated the sensitivity of Chinese hamster ovary (CHO) cells and human lung adenocarcinoma epithelial cell line (A549 cells) to TPM-induced cytotoxic effects. The results indicate that NRU and WST-1 assays are preferable to LDH activity assay for assessing the TPM-induced cytotoxicity, and NRU assay might be more sensitive than WST-1 assay. The cytotoxicity of 3R4F reference cigarettes and two commercial brands of cigarettes were tested by NRU assay in CHO and A549 cells. The results showed that EC₅₀ values in CHO cells treated with TPM were lower than EC₅₀ values in A549 cells, indicating CHO cells are more sensitive to TPM-induced cytotoxic effects than A549 cells.</p> </abstract>