Glycoengineering of host mimicking type‐2 LacNAc polymers and Lewis X antigens on bacterial cell surfaces. Issue 1 (30th November 2012)

• Record Type: Journal Article
• Authors: Mally, Manuela; Fontana, Carolina; LeibundGut‐Landmann, Salomé; Laacisse, Lamia; Fan, Yao‐Yun; Widmalm, Göran; Aebi, Markus
• Is Part Of: Molecular microbiology. Volume 87:Issue 1(2013)
• Page Start: 112
• Page End: 131
• Publication Date: 2012-11-30
• Subjects: Molecular microbiology -- Periodicals; 572.829
• DOI: 10.1111/mmi.12086
• Languages: English
• ISSNs: 0950-382X

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Bacterial carbohydrate structures play a central role in mediating a variety of host–pathogen interactions. Glycans can either elicit protective immune response or lead to escape of immune surveillance by mimicking host structures. Lipopolysaccharide (LPS), a major component on the surface of Gram‐negative bacteria, is composed of a lipid A‐core and the O‐antigen polysaccharide. Pathogens like <italic>Neisseria meningitidis</italic> expose a lipooligosaccharide (LOS), which outermost glycans mimick mammalian epitopes to avoid immune recognition. Lewis X (Galβ1–4(Fucα1–3)GlcNAc) antigens of <italic>Helicobacter pylori</italic> or of the helminth <italic>Schistosoma mansoni</italic> modulate the immune response by interacting with receptors on human dendritic cells. In a glycoengineering approach we generate human carbohydrate structures on the surface of recombinant Gram‐negative bacteria, such as <italic>Escherichia coli</italic> and <italic>Salmonella enterica</italic> sv. Typhimurium that lack O‐antigen. A ubiquitous building block in mammalian N‐linked protein glycans is Galβ1‐4GlcNAc, referred to as a type‐2 N‐acetyllactosamine, LacNAc, sequence. Strains displaying polymeric LacNAc were generated by introducing a combination of glycosyltransferases that act on modified lipid A‐cores, resulting in efficient expression of the carbohydrate epitope on bacterial cell surfaces. The poly‐LacNAc scaffold was used as an acceptor for fucosylation leading to polymers of Lewis X antigens. We analysed the distribution of the carbohydrate epitopes by FACS, microscopy and ELISA and confirmed engineered LOS containing LacNAc and Lewis X repeats by MALDI‐TOF and NMR analysis. Glycoengineered LOS induced pro‐inflammatory response in murine dendritic cells. These bacterial strains can thus serve as tools to analyse the role of defined carbohydrate structures in different biological processes.</p> </abstract>