Mesenchymal stem cells combined with barrier domes enhance vertical bone formation. (19th December 2012)

• Record Type: Journal Article
• Authors: Zigdon‐Giladi, Hadar; Lewinson, Dina; Bick, Tova; Machtei, Eli E.
• Is Part Of: Journal of clinical periodontology. Volume 40:Number 2(2013:Feb.)
• Page Start: 196
• Page End: 202
• Publication Date: 2012-12-19
• Subjects: Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.12044
• Languages: English
• ISSNs: 0303-6979

Abstract

<abstract abstract-type="main" id="jcpe12044-abs-0001"> <title>Abstract</title> <sec id="jcpe12044-sec-0001" sec-type="section"> <title>Aim</title> <p>To enhance vertical bone formation in a rat calvarium following combination of guided bone regeneration (GBR) and transplantation of bone marrow derived mesenchymal stem cells (bmMSC).</p> </sec> <sec id="jcpe12044-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>Gold domes (7 mm radius, 5 mm height) were filled with 5 × 10<sup>5</sup> bmMSC or osteogenic transformed bmMSC (otMSC) that were isolated from tibia of inbred rats and mixed with βTCP. Domes filled with βTCP served as control. Rats were sacrificed after 3 months. New bone formation was analysed by histology and histomorphometry.</p> </sec> <sec id="jcpe12044-sec-0003" sec-type="section"> <title>Results</title> <p>In all rats hard tissue filled the space under the dome. In the lower part of the specimens the newly formed mature bone was continuous with the original calvaria, whereas the upper (distal) part of the augmented tissue contained residual scaffold surrounded by connective tissue. Histomorphometric analysis revealed that cell transplantation doubled vertical bone height: (bmMSC 4.13 ± 0.5 mm, otMSC 4.14 ± 0.3 mm βTCP 2.29 ± 0.22 mm, <italic>p</italic> ≤ 0.001). Bone area fraction (%) was significantly increased following transplantation of otMSC (47.2 ± 2.5%) when compared with bmMSC (37.3 ± 3.35%) and with βTCP (31.09 ± 2.7%) (<italic>p</italic> ≤ 0.031 <italic>versus</italic> bmMSC, <italic> p</italic> ≤ 0.0004 <italic>versus</italic> control)</p> </sec> <sec id="jcpe12044-sec-0004" sec-type="section"> <title>Conclusion</title> <p>In a rat calvaria model transplantation of both otMSC and bmMSC, when combined with GBR significantly enhanced bone formation.</p> </sec> </abstract>