Molecular mechanism of action of bisphenol and bisphenol A mediated by oestrogen receptor alpha in growth and apoptosis of breast cancer cells. (12th April 2013)

• Record Type: Journal Article
• Authors: Sengupta, S; Obiorah, I; Maximov, PY; Curpan, R; Jordan, VC
• Is Part Of: British journal of pharmacology. Volume 169:Number 1(2013:May)
• Page Start: 167
• Page End: 178
• Publication Date: 2013-04-12
• Subjects: Pharmacology -- Periodicals; Chemotherapy -- Periodicals; Drug Therapy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1111/bph.12122
• Languages: English
• ISSNs: 0007-1188

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="bph12122-sec-0001" sec-type="section"> <title>Background and Purpose</title> <p>Oestrogen receptor alpha (ERα) binds to different ligand which can function as complete/partial oestrogen‐agonist or antagonist. This depends on the chemical structure of the ligands which modulates the transcriptional activity of the oestrogen‐responsive genes by altering the conformation of the liganded‐ERα complex. This study determined the molecular mechanism of oestrogen‐agonistic/antagonistic action of structurally similar ligands, bisphenol (BP) and bisphenol A (BPA) on cell proliferation and apoptosis of ERα + ve breast cancer cells.</p> </sec> <sec id="bph12122-sec-0002" sec-type="section"> <title>Experimental Approach</title> <p>DNA was measured to assess the proliferation and apoptosis of breast cancer cells. RT‐PCR and ChIP assays were performed to quantify the transcripts of <italic>TFF1</italic> gene and recruitment of ERα and SRC3 at the promoter of <italic>TFF1</italic> gene respectively. Molecular docking was used to delineate the binding modes of BP and BPA with the ERα. PCR‐based arrays were used to study the regulation of the apoptotic genes.</p> </sec> <sec id="bph12122-sec-0003" sec-type="section"> <title>Key Results</title> <p>BP and BPA induced the proliferation of breast cancer cells; however, unlike BPA, BP failed to induce apoptosis. BPA consistently acted as an agonist in our studies but BP exhibited mixed agonistic/antagonistic properties. Molecular docking revealed agonistic and antagonistic mode of binding for BPA and BP respectively. BPA treatment resembled E2 treatment in terms of PCR‐based regulation of apoptotic genes whereas BP was similar to 4OHT treatment.</p> </sec> <sec id="bph12122-sec-0004" sec-type="section"> <title>Conclusions and Implications</title> <p>The chemical structure of ERα ligand determines the agonistic or antagonistic biological responses by the virtue of their binding mode, conformation of the liganded‐ERα complex and the context of the cellular function.</p> </sec> </abstract>