Ectopic Expression of Rice OsBIANK1, Encoding an Ankyrin Repeat‐Containing Protein, in Arabidopsis Confers Enhanced Disease Resistance to Botrytis cinerea and Pseudomonas syringae. Issue 1 (26th September 2012)

• Record Type: Journal Article
• Authors: Li, Dayong; Wang, Fengrang; Liu, Bo; Zhang, Yafen; Huang, Lei; Zhang, Huijuan; Song, Fengming
• Is Part Of: Journal of phytopathology. Volume 161:Issue 1(2013:Jan.)
• Page Start: 27
• Page End: 34
• Publication Date: 2012-09-26
• Subjects: Plant diseases -- Periodicals; 632
• DOI: 10.1111/jph.12023
• Languages: English
• ISSNs: 0931-1785

Abstract

<abstract abstract-type="main" id="jph12023-abs-0001"> <title>Abstract</title> <p>Ankyrin repeat‐containing proteins comprise a large family whose members have been shown to play important roles in various aspects of biological processes in plant growth and development as well as in responses to biotic and abiotic stresses. We previously identified a rice gene, <italic>OsBIANK1</italic>, encoding an ankyrin repeat‐containing protein and found that expression of <italic>OsBIANK1</italic> can be induced by defence signalling molecules and by infection of <italic>Magnaporthe oryzae, </italic> the causal agent of blast disease. To better understand the possible function of <italic>OsBIANK1</italic> in disease resistance, we generated transgenic <italic>Arabidopsis</italic> plants that constitutively overexpress the <italic>OsBIANK1</italic> gene. Results from disease assays revealed that the <italic>OsBIANK1</italic>‐overexpressing plants display increased resistance against <italic>Botrytis cinerea</italic> and <italic>Pseudomonas syringae</italic> pv. <italic>tomato </italic>DC3000 as compared with the wild‐type plants. In <italic>OsBIANK1</italic>‐overexpressing plants, expression of some of well‐known defence genes (e.g. <italic>PR</italic>‐<italic>1</italic>, <italic> PR</italic>‐<italic>2</italic> and <italic>PDF1.2</italic>) was up‐regulated after infection with <italic>B. cinerea</italic> or <italic>P. syringae</italic> pv. <italic>tomato </italic>DC3000. Furthermore, the <italic>OsBIANK1</italic>‐overexpressing plants showed decreased levels of reactive oxygen species (i.e. superoxide anion and H<sub>2</sub>O<sub>2</sub>) after <italic>Botrytis</italic> infection. Thus, our present results further support the role of <italic>OsBIANK1</italic> in regulation of defence responses against different types of pathogens.</p> </abstract>