Residual antibacterial activity of dog hairs after therapy with antimicrobial shampoos. Issue 2 (22nd February 2013)

• Record Type: Journal Article
• Authors: Kloos, Isabell; Straubinger, Reinhard K.; Werckenthin, Christiane; Mueller, Ralf S.
• Is Part Of: Veterinary dermatology. Volume 24:Issue 2(2013:Apr.)
• Page Start: 250
• Page End: e54
• Publication Date: 2013-02-22
• Subjects: Veterinary dermatology -- Periodicals; Pet medicine -- Periodicals; 636.08965
• DOI: 10.1111/vde.12012
• Languages: English
• ISSNs: 0959-4493

Abstract

<abstract abstract-type="main" xml:lang="en" id="vde12012-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="vde12012-sec-0001" sec-type="section"> <title>Background</title> <p>Topical antimicrobial treatment for canine pyoderma is becoming increasingly important, but little is known about the mechanism of action and persistence of activity of antimicrobial shampoos.</p> </sec> <sec id="vde12012-sec-0002" sec-type="section"> <title>Objective</title> <p>To determine the residual antimicrobial activity on canine hairs treated with antimicrobial shampoos.</p> </sec> <sec id="vde12012-sec-0003" sec-type="section"> <title>Animals</title> <p>Forty‐two dogs from a research institution.</p> </sec> <sec id="vde12012-sec-0004" sec-type="section"> <title>Methods</title> <p>Dogs were treated with six different shampoos and the combination of one shampoo and conditioner containing benzoyl peroxide, chlorhexidine in different concentrations (0.8, 2, 3 and 4%), ethyl lactate and miconazole twice weekly for 2 weeks. A shampoo vehicle without antimicrobial ingredients was used as the control. Hairs were collected immediately after and 2, 4 and 7 days after the last shampoo therapy and placed onto an agar plate streaked with <italic>Staphylococcus pseudintermedius</italic>. After incubation, the growth inhibition zone around the hair shafts was measured.</p> </sec> <sec id="vde12012-sec-0005" sec-type="section"> <title>Results</title> <p>The largest zone of inhibition of bacterial growth was seen after shampoos containing 2 and 3% chlorhexidine and the combination of chlorhexidine shampoo and conditioner. The zone of inhibition was smaller with the shampoos containing 0.8 and 4% chlorhexidine. There was no difference between the inhibition zones post‐treatment with benzoyl peroxide, ethyl lactate and control.</p> </sec> <sec id="vde12012-sec-0006" sec-type="section"> <title>Conclusion and clinical importance</title> <p>The efficacy of a shampoo is dependent not only on the concentration of the active ingredients but also on the shampoo formulation. Hair shafts treated with shampoos containing 2 and 3% chlorhexidine and the combination of shampoo and conditioner inhibited bacterial growth significantly and seem suitable to treat canine bacterial pyoderma.</p> </sec> </abstract>