Mass spectrometry imaging of freeze‐dried membrane phospholipids of dividing Tetrahymena pyriformis. (14th June 2012)

• Record Type: Journal Article
• Authors: Lanekoff, Ingela; Phan, Nhu TN.; Van Bell, Craig T.; Winograd, Nicholas; Sjövall, Peter; Ewing, Andrew G.
• Is Part Of: Surface and interface analysis. Volume 45:Number 1(2013:Jan.)
• Page Start: 211
• Page End: 214
• Publication Date: 2012-06-14
• Subjects: Surfaces (Physics) -- Periodicals; Surface chemistry -- Periodicals; Thin films -- Periodicals; 541.33
• DOI: 10.1002/sia.5017
• Languages: English
• ISSNs: 0142-2421

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Time of Flight secondary ion mass spectrometry (TOF‐SIMS) has been used to explore the distribution of phospholipids in the plasma membrane of <italic>Tetrahymena pyriformis</italic> during cell division. The dividing cells were freeze‐dried prior to analysis followed by line scan and region of interest analysis at various stages of cell division. The results showed no signs of phospholipid domain formation at the junction between the dividing cells. Instead the results showed that the sample preparation technique had a great impact on one of the examined phospholipids, namely phosphatidylcholine (PC). Phosphatidylcholine and 2‐aminoethylphosphonolipid (2‐AEP) have therefore been evaluated in <italic>Tetrahymena</italic> cells that have been subjected to different sample preparation techniques: freeze drying <italic>ex situ</italic>, freeze fracture, and freeze fracture with partial or total freeze drying <italic>in situ</italic>. The result suggests that freeze drying <italic>ex situ</italic> causes the celia to collapse and cover the plasma membrane. Copyright © 2012 John Wiley &amp; Sons, Ltd.</p> </abstract>