Effect of tea catechins on regulation of cell proliferation and antioxidant enzyme expression in H2O2‐induced primary hepatocytes of goat in vitro. Issue 3 (14th March 2012)

• Record Type: Journal Article
• Authors: Zhong, R. Z.; Xiao, W. J.; Zhou, D. W.; Tan, C. Y.; Tan, Z. L.; Han, X. F.; Zhou, C. S.; Tang, S. X.
• Is Part Of: Journal of animal physiology and animal nutrition. Volume 97:Issue 3(2013:Jun.)
• Page Start: 475
• Page End: 484
• Publication Date: 2012-03-14
• Subjects: Animal nutrition -- Periodicals; Feeds -- Periodicals; 636.085
• DOI: 10.1111/j.1439-0396.2012.01288.x
• Languages: English
• ISSNs: 0931-2439

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Summary</title> <p>Tea catechins (TC) are polyphenols that have potent antioxidant activity. The objectives of this study were to determine the effects of TC on antioxidant status of hepatocytes challenged with H<sub>2</sub>O<sub>2</sub>. Primary hepatocytes of goat were exposed to 1 m<sc>m</sc> H<sub>2</sub>O<sub>2</sub> without or with 5, 50 and 500 μg/ml TC. The cells were harvested at 48 h post‐treatment to determine effects of TC on proliferation, apoptotic features and membrane integrity of cells, and expression of genes and activities of antioxidant enzymes. H<sub>2</sub>O<sub>2</sub> exposure caused damage to cells (p<italic> </italic><<italic> </italic>0.001). A lower concentration of TC (5 μg/ml) displayed a protective effect by inhibiting exorbitant cell proliferation and DNA degradation. Both H<sub>2</sub>O<sub>2</sub> exposure and TC pre‐incubation affected expression of antioxidant enzymes at mRNA and protein levels (p<italic> </italic><<italic> </italic>0.001). The activities of catalase (CAT) (p<italic> </italic>=<italic> </italic>0.027), CuZn‐superoxide dismutase (CuZn‐SOD) (p<italic> </italic><<italic> </italic>0.001) and glutathione peroxidase (GPx) (p<italic> </italic><<italic> </italic>0.001) increased with TC pre‐incubation followed by H<sub>2</sub>O<sub>2</sub> challenge. Changes of CuZn‐SOD activity induced by H<sub>2</sub>O<sub>2</sub> and TC basically paralleled the changes in the corresponding mRNA and protein levels, but the correlation in CAT and GPx expression displayed slightly different patterns at different concentrations of TC. These findings infer that oxidative stress can induce deleterious cellular responses and this unfavourable condition may be alleviated by treatment with TC.</p> </abstract>