CD4+ T cells producing interleukin (IL)‐17, IL‐22 and interferon‐γ are major effector T cells in nickel allergy. (22nd May 2013)

• Record Type: Journal Article
• Authors: Dyring‐Andersen, Beatrice; Skov, Lone; Løvendorf, Marianne B.; Bzorek, Michael; Søndergaard, Knud; Lauritsen, Jens‐Peter H.; Dabelsteen, Sally; Geisler, Carsten; Menné Bonefeld, Charlotte
• Is Part Of: Contact dermatitis. Volume 68:Number 6(2013:Jun.)
• Page Start: 339
• Page End: 347
• Publication Date: 2013-05-22
• Subjects: Contact dermatitis -- Periodicals; 616.51
• DOI: 10.1111/cod.12043
• Languages: English
• ISSNs: 0105-1873

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Summary</title> <p> <bold>Background</bold> It has been suggested that interleukin (IL)‐17 and IL‐22 play important roles in the elicitation of human allergic contact dermatitis; however, the frequencies of T cell subtypes producing IL‐17 and IL‐22 in human allergic contact dermatitis are unknown.</p> <p> <bold>Objectives</bold> To determine the frequencies of CD4<sup>+</sup>, CD8<sup>+</sup> and <italic>γδ</italic> T cells producing IL‐17, IL‐22 and interferon (IFN)‐<italic>γ</italic> in the blood and skin from nickel‐allergic patients.</p> <p> <bold>Patients/materials/methods</bold> Blood samples were collected from 14 patients and 17 controls, and analysed by flow cytometry. Biopsies were taken from 5 patients and 6 controls, and analysed by immunohistochemistry and flow cytometry of skin lymphocytes.</p> <p> <bold>Results</bold> We found an increased frequency of <italic>γδ</italic> T cells in the blood, but no differences in the distribution of cytokine‐producing CLA<sup>+</sup> T cell subtypes in nickel‐allergic patients as compared with controls. In nickel‐allergic patients, there was massive cellular infiltration dominated by CD4<sup>+</sup> T cells producing IL‐17, IL‐22 and IFN‐<italic>γ</italic> in nickel‐challenged skin but not in vehicle‐challenged skin.</p> <p> <bold>Conclusion</bold> CD4<sup>+</sup> T cells producing IL‐17, IL‐22 and IFN‐<italic>γ</italic> are important effector cells in the eczematous reactions of nickel‐induced allergic contact dermatitis in humans.</p> </abstract>