Human periodontal ligament cells reaction on a novel hydroxyapatite–collagen scaffold. Issue 2 (8th June 2012)

• Record Type: Journal Article
• Authors: Guo, Jing; Wang, Ying; Cao, Chengbo; Dziak, Rosemary; Preston, Brian; Guan, Guoqiang
• Is Part Of: Dental traumatology. Volume 29:Issue 2(2013:Apr.)
• Page Start: 103
• Page End: 109
• Publication Date: 2012-06-08
• Subjects: Teeth -- Wounds and injuries -- Periodicals; Dentistry, Operative -- Periodicals; Traumatology -- Periodicals; Endodontics -- Periodicals; 617.6
• DOI: 10.1111/j.1600-9657.2012.01152.x
• Languages: English
• ISSNs: 1600-4469

Abstract

<abstract abstract-type="main" id="edt1152-abs-0001"> <title>Abstract</title> <sec id="edt1152-sec-0001" sec-type="section"> <title>Background</title> <p>Periodontal tissue regeneration presents a highly promising method for restoring periodontal structures. The development of a suitable bioactive scaffold that promotes cell proliferation and differentiation is critical in periodontal tissue engineering. The aim of this study was to evaluate the biocompatibility of a novel 3‐dimensional hydroxyapatite–collagen scaffold with human periodontal ligament (hPDL) cell culture.</p> </sec> <sec id="edt1152-sec-0002" sec-type="section"> <title>Methods</title> <p>The scaffold was produced from a natural collagen matrix ‐ purified porcine acellular dermal matrix (PADM), which was then treated with hydroxyapatite (HA) through a biomimetic chemical process to obtain hydroxyapatite–porcine acellular dermal matrix (HA‐PADM) scaffold. The hPDL cells were cultured with HA‐PADM scaffolds for 1, 3, 6, 14, and 28 days. The cell viability assay, scanning electron microscopy (SEM), hematoxylin and eosin (H&E) staining, immunohistochemistry, and confocal microscopy were employed in different time points to evaluate the biocompatibility of the scaffolds with hPDL cells.</p> </sec> <sec id="edt1152-sec-0003" sec-type="section"> <title>Results</title> <p>The cell viability assay (WST‐1 test) verified cell proliferation on the HA‐PADM scaffolds. The SEM study showed unique morphology of hPDL cells, which attach and spread on the surface of the scaffolds. The H&E staining, immunohistochemistry, and confocal microscopy demonstrated that hPDL cells were able to grow into the HA‐PADM scaffolds and maintain viability after prolonged culture.</p> </sec> <sec id="edt1152-sec-0004" sec-type="section"> <title>Conclusions</title> <p>This study proved that HA‐PADM scaffold is ‐biocompatible for hPDL cells. The cells were able to proliferate and migrate into the scaffold. These observations suggest that HA‐PADM is a potential cell carrier for periodontal tissue regeneration.</p> </sec> </abstract>