Character comparison of abdomen‐derived and eyelid‐derived mesenchymal stem cells. (22nd May 2013)

• Record Type: Journal Article
• Authors: Kim, S.‐H.; Bang, S. H.; Park, S. A.; Kang, S. Y.; Park, K. D.; Oh, I. U.; Yoo, S. H.; Kim, H.; Kim, C.‐H.; Baek, S. Y.
• Is Part Of: Cell proliferation. Volume 46:Number 3(2013:Jun.)
• Page Start: 291
• Page End: 299
• Publication Date: 2013-05-22
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12027
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" xml:lang="en" id="cpr12027-abs-0001"> <title>Abstract</title> <sec id="cpr12027-sec-0001" sec-type="section"> <title>Objectives</title> <p>While most human adipose tissues, such as those located in the abdomen, hip and thigh, are of mesodermal origin, adipose tissues located in the face are of ectodermal origin. The present study has compared stem cell‐related features of abdomen‐derived adult stem cells (A‐ASCs) with those of eyelid‐derived adult stem cells (E‐ASCs).</p> </sec> <sec id="cpr12027-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Adipose tissue‐derived cells were maintained in DMEM supplemented with 10% FBS. Before passage 6, cells were analysed using FACS, immunocytochemistry and quantitative real time PCR (qRT‐PCR). To examine multi‐differentiational potential, early passage ASCs were cultivated in each of a commercial Stempro<sup>®</sup> Differentiation kit.</p> </sec> <sec id="cpr12027-sec-0003" sec-type="section"> <title>Results</title> <p>Unlike fibroblast‐like morphology of A‐ASCs, E‐ASCs had bipolar morphology. Both types of cell exhibited similar surface antigens, and neuronal cell‐related genes and proteins. However, there were differences in mRNA expression levels of CD90 and CD146; neuron‐specific enolase (NSE) and nuclear receptor‐related protein 1 (Nurr1) were different between the two cell types. There was no difference in multi‐differentiational potential between 3 E‐ASCs lines, however, E‐ASCs had higher expression levels of chondrocyte‐related genes compared to A‐ASCs. These cells underwent senescence and maintained normal karyotypes.</p> </sec> <sec id="cpr12027-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Although isolated from similar adipose tissues, both types of cells displayed many contrasting characteristics. Understanding defining phenotypes of such cells is useful for making suitable choices in differing clinical indications.</p> </sec> </abstract>