Proposed vector candidate: Leptotrombidium palpale for Shimokoshi type Orientia tsutsugamushi. (12th February 2013)

• Record Type: Journal Article
• Authors: Seto, Junji; Suzuki, Yu; Otani, Katsumi; Qiu, Yongjin; Nakao, Ryo; Sugimoto, Chihiro; Abiko, Chieko
• Is Part Of: Microbiology and immunology. Volume 57:Number 2(2013:Feb.)
• Page Start: 111
• Page End: 117
• Publication Date: 2013-02-12
• Subjects: Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12015
• Languages: English
• ISSNs: 0385-5600

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="mim12015-sec-0001" sec-type="section"> <p>To identify the vector species for Shimokoshi type <italic>Orientia tsutsugamushi</italic>, a survey of larval trombiculid mites was conducted in Yamagata Prefecture, Japan from April to May 2012. In all, 2889 larval trombiculid mites were obtained from 21 <italic>Apodemus speciosus</italic> rodent hosts, 2600 of which were morphologically classified into eight species in three genera. After screening of <italic>O. tsutsugamushi</italic> DNA in individual larval trombiculid mites using real‐time PCR targeting the 16S ribosomal RNA gene, serotype‐specific nested PCRs targeting the 56 kDa protein gene were performed, followed by sequencing analysis. As a result, Shimokoshi type <italic>O. tsutsugamushi</italic> DNA was identified from 3 (1.9%) of 157 <italic>Leptotrombidium palpale</italic>. This is the first study to identify Shimokoshi type <italic>O. tsutsugamushi</italic> DNA in <italic>L. palpale</italic>. The results indicate that <italic>L. palpale</italic> is a possible vector for Shimokoshi type <italic>O. tsutsugamushi</italic>.</p> </sec> </abstract>