Antimicrobial effectiveness of intracanal medicaments on Enterococcus faecalis: chlorhexidine versus octenidine. (30th June 2012)

• Record Type: Journal Article
• Authors: de, J. M. V. M.; Decker, E. M.; Walter, C.; Boeira, L. S.; Löst, C.; Weiger, R.
• Is Part Of: International endontic journal. Volume 46:Number 1(2013:Jan.)
• Page Start: 53
• Page End: 61
• Publication Date: 2012-06-30
• Subjects: Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/j.1365-2591.2012.02093.x
• Languages: English
• ISSNs: 0143-2885

Abstract

<abstract abstract-type="main" id="iej2093-abs-0001"> <title>Abstract</title> <sec id="iej2093-sec-0001" sec-type="section"> <title>Aim</title> <p>To determine the viability of <italic> Enterococcus faecalis</italic> in infected human root dentine <italic>in vitro</italic> after exposure to root canal medicaments based on chlorhexidine and octenidine.</p> </sec> <sec id="iej2093-sec-0002" sec-type="section"> <title>Methodology</title> <p>Human root segments (<italic>n</italic> = 40) were infected with <italic> E. faecalis</italic> for 8 weeks. Root dentine samples (rd) collected at week 4 served as individual baseline values. At week 8, the root segments were randomly divided into four test groups (<italic>n</italic> = 10 each) for the placement of one of the following medicaments in the root canals: calcium hydroxide paste (CH), chlorhexidine gel (CHX‐gel) (5.0%), chlorhexidine/gutta‐percha points (CHX‐GP) (active points<sup>®</sup>; Roeko, Langenau, Germany) and octenidine gel (OCT‐gel) (5.0%) followed by incubation for 4 weeks. The effect on <italic> E. faecalis</italic> viability was assessed by two fluorescent dyes (syto 9/propidium iodide) to determine the 'proportion of viable bacteria' (PVB%) and number of 'colony‐forming units' (CFU). Mean values and 95% confidence intervals (CI) were calculated for PVB% and log CFU, and the difference between groups was established.</p> </sec> <sec id="iej2093-sec-0003" sec-type="section"> <title>Results</title> <p>Viable and dead bacterial cells were detected in all 'rd' samples at weeks 4 and 8. The treatment with CHX‐gel, CHX‐GP and OCT‐gel resulted in significantly lower PVB% values with 15.4%, 3.5% and 0%, respectively. No growth (CFU) was recorded for these samples at week 12. When medicated by CH, the PVB% was increased without a corresponding change in CFUs.</p> </sec> <sec id="iej2093-sec-0004" sec-type="section"> <title>Conclusions</title> <p>In contrast to calcium hydroxide, both CHX – and octenidine‐based intracanal medicaments were effective in decreasing the viability of <italic> E. faecalis</italic>. OCT showed the most favourable results and may have potential as an endodontic medicament.</p> </sec> </abstract>