PER3 and ADORA2A polymorphisms impact neurobehavioral performance during sleep restriction. (21st November 2012)

• Record Type: Journal Article
• Authors: Rupp, Tracy L.; Wesensten, Nancy J.; Newman, Rachel; Balkin, Thomas J.
• Is Part Of: Journal of sleep research. Volume 22:Number 2(2013:Jun.)
• Page Start: 160
• Page End: 165
• Publication Date: 2012-11-21
• Subjects: Sleep -- Periodicals; Sleep disorders -- Periodicals; 612.821
• DOI: 10.1111/j.1365-2869.2012.01062.x
• Languages: English
• ISSNs: 0962-1105

Abstract

<abstract abstract-type="main" id="jsr1062-abs-0001"> <title>Summary</title> <p>The objective of the study was to determine whether <italic>ADORA2A</italic> or <italic>PER3</italic> polymorphisms contribute to individual responsivity to sleep restriction. Nineteen healthy adults (ages 18–39, 11 males, 8 females) underwent sleep restriction (SR) which consisted of seven nights of 3 h time in bed (TIB) (04:00–07:00). SR was preceded by seven in‐laboratory nights of 10 h TIB (21:00–07:00) and followed by three nights of 8 h TIB (23:00–07:00). Volunteers underwent psychomotor vigilance, objective alertness, and subjective sleepiness assessments throughout. Volunteers were genotyped for the <italic>PER3 </italic>VNTR polymorphism (<italic>PER3</italic><sup>4/4</sup><italic>n</italic> = 7; <italic>PER3</italic><sup>4/5</sup><italic>n</italic> = 10; <italic>PER3</italic><sup>5/5</sup><italic>n</italic> = 2) and the <italic>ADORA2A</italic> c.1083T>C polymorphism, (<italic>ADORA2</italic><italic>A</italic><sup>C</sup><sup>/T</sup><italic>n</italic> = 9; <italic>ADORA2</italic><italic>A</italic><sup>T</sup><sup>/T</sup><italic>n</italic> = 9; <italic>ADORA2</italic><italic>A</italic><sup>C</sup><sup>/C</sup><italic>n</italic> = 1) using polymerase chain reaction (PCR). Separate mixed‐model <sc>anova</sc>s were used to assess contributions of <italic>ADORA2A</italic> and <italic>PER3</italic> polymorphisms. Results showed that <italic>PER3</italic><sup>4/4</sup> and <italic>ADORA2A</italic><sup>C/T</sup> individuals expressed greater behavioral resiliency to SR compared to PER<sup>4/5</sup>and <italic>ADORA2A</italic><sup>T/T</sup> individuals. Our findings contrast with previously reported non‐significant effects for the <italic>PER3</italic> polymorphism under a less challenging sleep restriction regimen (4 h TIB per night for five nights). We conclude that <italic>PER3</italic> and <italic>ADORA2A</italic> polymorphisms become more behaviorally salient with increasing severity and/or duration of sleep restriction (based on psychomotor vigilance). Given the small sample size these results are preliminary and require replication.</p> </abstract>