Development and validation of a sensitive LC/MS/MS method for the determination of γ‐tocotrienol in rat plasma: application to pharmacokinetic studies. (20th April 2012)

• Record Type: Journal Article
• Authors: Liang, Su; Bian, Xiaomei; Ma, Jing; Arogunjo, Motolani; Deorukhkar, Amit A.; Krishnan, Sunil; Xie, Huan
• Is Part Of: Biomedical chromatography. Volume 27:Number 1(2013:Jan.)
• Page Start: 58
• Page End: 66
• Publication Date: 2012-04-20
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.2748
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p> <italic>γ</italic>‐Tocotrienol has attracted much attention owing to its multiple health benefits. This study developed and validated a simple, specific, sensitive and reliable LC/MS/MS method to analyze <italic>γ</italic>‐tocotrienol in rat plasma. Plasma samples (50 μL) were extracted with internal standard solution (25 ng/mL of itraconazole) in acetonitrile (200 μL) with an average recovery of 44.7% and an average matrix effect of −2.9%. The separation of <italic>γ</italic>‐tocotrienol and internal standard from the plasma components was achieved with a Waters XTerra® MS C<sub>18</sub> column with acetonitrile–water as mobile phase. Analysis was performed under positive ionization electrospray mass spectrometer via the multiple reaction monitoring. The standard curve was linear over a concentration range of 10–1000 ng/mL with correlation coefficient values >0.997. The method was validated with intra‐ and inter‐day accuracy (relative error) ranging from 1.79 to 9.17% and from 2.16 to 9.66%, respectively, and precision (coefficient of variation) ranged from 1.94 to 9.25% and from 2.37 to 10.08%, respectively. Short‐term stability, freeze–thaw stability and the processed sample stability tests were performed. This method was further applied to analyze <italic>γ</italic>‐tocotrienol plasma concentrations in rats at various time points after administration of a 2 mg/kg single intravenous dose, and a pharmacokinetic profile was successfully obtained. Copyright © 2012 John Wiley & Sons, Ltd.</p> </abstract>