A sensitive flow injection chemiluminescence method for the determination of progesterone. Issue 4 (14th June 2011)

• Record Type: Journal Article
• Authors: Cao, Wei; Gong, Pixue; Liu, Wenwen; Zhuang, Mei; Yang, Jinghe
• Is Part Of: Drug testing and analysis. Volume 5:Issue 4(2013:Apr.)
• Page Start: 242
• Page End: 246
• Publication Date: 2011-06-14
• Subjects: Drugs -- Analysis -- Periodicals; Drug testing -- Periodicals; Chemistry, Forensic -- Periodicals; 615.1901
• DOI: 10.1002/dta.299
• Languages: English
• ISSNs: 1942-7603

Abstract

<abstract abstract-type="main" xml:lang="en" id="dta299-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="dta299-para-0001">A sensitive flow injection (FI) chemiluminescence (CL) method was developed for the determination of trace amounts of progesterone. This method was based on the luminescent properties of the tris(1, 10‐phenanthroline) ruthenium(II) ‐ potassium permanganate (KMnO₄) ‐ progesterone in acidic medium sensitized by Na₂SO₃. With the peak height as a quantitative parameter applying optimum conditions, the relative CL intensity was linear with progesterone concentration in the range of 1.0 × 10⁻¹⁰ ∼ 6.0 × 10⁻⁹ g·ml⁻¹ and 6.0 × 10⁻⁹ ∼ 4.0 × 10⁻⁸ g·ml⁻¹ with a detection limit of 7.1 × 10⁻¹¹ g·ml⁻¹. The relative standard deviation (RSD) was 2.79% for 1.0 × 10⁻⁸ g·ml⁻¹ progesterone (<italic>n</italic> = 11). The proposed method held low detection limit and was successfully applied to determination of progesterone in pharmaceutical preparations. The possible CL reaction mechanism was also discussed. Copyright © 2011 John Wiley &amp; Sons, Ltd.</p> </abstract>