Establishment of medium for laboratory cultivation and maintenance of Fredericella sultana for in vivo experiments with Tetracapsuloides bryosalmonae (Myxozoa). Issue 2 (5th November 2012)

• Record Type: Journal Article
• Authors: Kumar, G; Abd‐Elfattah, A; Soliman, H; El‐Matbouli, M
• Is Part Of: Journal of fish diseases. Volume 36:Issue 2(2013:Feb.)
• Page Start: 81
• Page End: 88
• Publication Date: 2012-11-05
• Subjects: Fishes -- Diseases -- Periodicals; 639.3
• DOI: 10.1111/j.1365-2761.2012.01440.x
• Languages: English
• ISSNs: 0140-7775

Abstract

<abstract abstract-type="main" id="jfd1440-abs-0001"> <title>Abstract</title> <p>The freshwater bryozoan <italic>Fredericella sultana</italic> (Blumenbach) is the most common invertebrate host of the myxozoan parasite <italic>Tetracapsuloides bryosalmonae</italic>, the causative agent of proliferative kidney disease in salmonid fish. Culture media play an important role in hatching of statoblasts and maintaining clean bryozoan colonies for Malacosporea research. We developed a novel culture medium, Bryozoan Medium C (BMC), for the cultivation and maintenance of <italic>F</italic>. <italic>sultana</italic> under laboratory conditions. Statoblasts of <italic>F. sultana</italic> were successfully hatched to produce transparent‐walled, specific pathogen‐free (SPF) colonies that were maintained &gt;12 months in BMC at pH 6.65. <italic>Tetracapsuloides bryosalmonae</italic> was successfully transmitted from infected brown trout, <italic>Salmo trutta </italic>L., to newly hatched <italic>F. sultana</italic> colonies in BMC, then from the infected bryozoan to SPF brown trout. This study demonstrated the utility of BMC (pH 6.65) for hatching statoblasts, long‐term cultivation of clean and transparent bryozoan colonies and maintenance of the <italic>Tetracapsuloides bryosalmonae</italic> life cycle in the laboratory for molecular genetic research and other studies such as host–parasiteinteraction.</p> </abstract>