Sheep model for osteoporosis: Sustainability and biomechanical relevance of low turnover osteoporosis induced by hypothalamic–pituitary disconnection12. Issue 7 (25th February 2013)

• Record Type: Journal Article
• Authors: Oheim, Ralf; Beil, Frank Timo; Köhne, Till; Wehner, Tim; Barvencik, Florian; Ignatius, Anita; Amling, Michael; Clarke, Iain J.; Pogoda, Pia
• Is Part Of: Journal of orthopaedic research. Volume 31:Issue 7(2013:Jul.)
• Page Start: 1067
• Page End: 1074
• Publication Date: 2013-02-25
• Subjects: Orthopedics -- Periodicals; Musculoskeletal system -- Periodicals; 616.7
• DOI: 10.1002/jor.22327
• Languages: English
• ISSNs: 0736-0266

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jor22327-sec-0001" sec-type="section"> <p>Hypothalamo‐pituitary disconnection (HPD) leads to low bone turnover and osteoporosis in sheep. To determine the sustainability of bone loss and its biomechanical relevance, we studied HPD‐sheep 24 months after surgery (<italic>HPD</italic> + <italic>OVX‐24</italic>) in comparison to untreated control (<italic>Control</italic>), ovariectomized sheep (<italic>OVX</italic>), and sheep 12 months after HPD (<italic>HPD</italic> + <italic>OVX‐12</italic>). We performed histomorphometric, HR‐pQCT, and qBEI analyses, as well as biomechanical testing of all ewes studied. Twenty‐four months after HPD, histomorphometric analyses of the iliac crest showed a significant reduction of BV/TV by 60% in comparison to <italic>Control</italic>. Cortical thickness of the femora measured by HR‐pQCT did not change between 12 and 24 months after HPD but remained decreased by 30%. These structural changes were caused by a persisting depression of osteoblast and osteoclast cellular activity. Biomechanical testing of the femora showed a significant reduction of bending strength, whereas calcium content and distribution was found to be unchanged. In conclusion, HPD surgery leads to a persisting low turnover status with negative turnover balance in sheep followed by dramatic cortical and trabecular bone loss with consequent biomechanical impairment. © 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1067–1074, 2013</p> </sec> </abstract>