Effect of peginterferon alfa‐2a (40KD) on cytochrome P450 isoenzyme activity. (10th January 2013)

• Record Type: Journal Article
• Authors: Brennan, Barbara J.; Xu, Zhi‐Xin; Grippo, Joseph F.
• Is Part Of: British journal of clinical pharmacology. Volume 75:Number 2(2013:Feb.)
• Page Start: 497
• Page End: 506
• Publication Date: 2013-01-10
• Subjects: Pharmacology -- Periodicals; Drugs -- Periodicals; 615.1
• DOI: 10.1111/j.1365-2125.2012.04373.x
• Languages: English
• ISSNs: 0306-5251

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="bcp4373-sec-0001" sec-type="section"> <title>Aim</title> <p>Pegylated interferon‐based therapy is recommended for treatment of hepatitis C virus (HCV) infection. Because interferons are known to down‐regulate hepatic cytochrome P450 (CYP) enzymes, which are involved in drug metabolism and clearance, there is a need to investigate the effect of peginterferon (PEG‐IFN) alfa‐2a (40KD) on the activity of these enzymes <italic>in vivo</italic>.</p> </sec> <sec id="bcp4373-sec-0002" sec-type="section"> <title>Methods</title> <p>Fourteen healthy, male volunteers aged 18 to 45 years were recruited into an open label, two period, single centre study in which CYP enzyme activity was measured by administration of the selectively metabolized probe drugs theophylline (CYP1A2), tolbutamide (CYP2C9), mephenytoin (CYP2C19), debrisoquine (CYP2D6) and dapsone (CYP3A4) on day 1 of the study. PEG‐IFN alfa‐2a (40KD) 180 μg was given subcutaneously each week from day 15 to 36, and probe drugs were re‐administered on day 37. Probe drugs and metabolites were quantified in plasma or urine samples and used to derive pharmacokinetic parameters.</p> </sec> <sec id="bcp4373-sec-0003" sec-type="section"> <title>Results</title> <p>PEG‐IFN alfa‐2a (40KD) significantly increased the area under the serum drug concentration <italic>vs.</italic> time curve (AUC(0, ∞)) for theophylline by 24%, with a reduction in the mean oral clearance of theophylline of 20%. There were no effects on the pharmacokinetics of any of the other probe drugs. The incidence of adverse events was as expected in subjects receiving pegylated interferon.</p> </sec> <sec id="bcp4373-sec-0004" sec-type="section"> <title>Conclusion</title> <p>These results suggest there may be an inhibitory effect of PEG‐IFN alfa‐2a (40KD) on CYP1A2. PEG‐IFN alfa‐2a (40KD) had no effect on CYP2C9, CYP2C19, CYP2D6 or CYP3A4 in healthy subjects.</p> </sec> </abstract>