Optimal Imaging of In Vitro Clot Sonothrombolysis by MR‐Guided Focused Ultrasound. Issue 2 (14th November 2011)

• Record Type: Journal Article
• Authors: Durst, Christopher; Monteith, Stephen; Sheehan, Jason; Moldovan, Krisztina; Snell, John; Eames, Matt; Huerta, Thomas; Walker, William; Viola, Francesco; Kassell, Neal; Wintermark, Max
• Is Part Of: Journal of neuroimaging. Volume 23:Issue 2(2013)
• Page Start: 187
• Page End: 191
• Publication Date: 2011-11-14
• Subjects: Diagnostic imaging -- Periodicals; Nervous system -- Diseases -- Diagnosis -- Periodicals; Imagerie pour le diagnostic -- Périodiques; Système nerveux -- Maladies -- Diagnostic -- Périodiques; Imagerie médicale; Neuroimagerie; Neurologie; Système nerveux; Périodique électronique (Descripteur de forme); Ressource Internet (Descripteur de forme); 616.804754
• DOI: 10.1111/j.1552-6569.2011.00662.x
• Languages: English
• ISSNs: 1051-2284

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="abs1-1" sec-type="section"> <title>ABSTRACT</title> </sec> <sec id="abs1-2" sec-type="section"> <title>BACKGROUND AND PURPOSE</title> <p>As magnetic resonance‐guided focused ultrasound (MRgFUS) sonothrombolysis relies on mechanical rather than thermal mechanisms to achieve clot lysis, thermometry is not useful for the intraoperative monitoring of clot breakdown by MRgFUS. Therefore, the purpose of this study was to evaluate the optimum imaging sequence for sonothrombolysis.</p> </sec> <sec id="abs1-3" sec-type="section"> <title>METHODS</title> <p>In vitro blood drawn from 6 healthy volunteers was imaged using T1, T2 spin‐echo, and T2 gradient‐echo (GRE) sequences both before and after sonication using an Insightec ExAblate 4000 FUS transducer. Signal intensities of the three MR imaging sequences were measured and normalized to background signal for each time point. Representative samples of the pre‐ and postsonication clot were also sent to pathology for hematologic analysis.</p> </sec> <sec id="abs1-4" sec-type="section"> <title>RESULTS</title> <p>After sonication, the clot in the treatment tube was fully lysed as evidenced by physical and hematologic evaluation. The difference between pre‐ and postsonicated normalized signal intensity ratios demonstrated statistical significance only on T2 and GRE sequences (<italic>P</italic> < .001). However, significant blooming artifact limited interpretation on all GRE images.</p> </sec> <sec id="abs1-5" sec-type="section"> <title>CONCLUSION</title> <p>T2 is the most appropriate sequence for the evaluation of mechanical MRgFUS sonothrombolysis of an in vitro clot. These findings are consistent across the oxidative states of clot up to 48 hours.</p> </sec> </abstract>