Low‐dose probenecid selectively inhibits urinary excretion of phenolsulfonphthalein in rats without affecting biliary excretion. Issue 6 (7th December 2011)

• Record Type: Journal Article
• Authors: Shin, Yong‐Jun; Lee, Joo Hyun; Oh, Ju‐Hee; Lee, Young‐Joo
• Is Part Of: Journal of applied toxicology. Volume 33:Issue 6(2013)
• Page Start: 511
• Page End: 515
• Publication Date: 2011-12-07
• Subjects: Toxicology -- Periodicals; Industrial toxicology -- Periodicals; Environmentally induced diseases -- Periodicals; Toxicology -- Periodicals; 615.9005
• DOI: 10.1002/jat.1778
• Languages: English
• ISSNs: 0260-437X

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Renal organic anion transport systems play an important role in the excretion of anionic drugs and toxic compounds. Probenecid has been used as a potent inhibitor of urinary and biliary excretion of anionic compounds mediated by transporters such as organic anion transporters and multidrug resistance‐associated protein 2 (Mrp2). The purpose of this study was to optimize the dose of probenecid required for selective inhibition of urinary excretion of anionic compounds in rats, without inhibition of biliary excretion. Phenolsulfonphthalein (PSP), a model anionic compound that is excreted in urine and bile, was intravenously administered to rats after intraperitoneal injection of different doses of probenecid (0, 0.2, 2, 10, 100, 200 and 400 mg kg<sup>−1</sup>). Treatment with 100, 200 or 400 mg kg<sup>−1</sup> probenecid decreased both renal clearance (<italic>CL</italic><sub>r</sub>) and biliary clearance (<italic>CL</italic><sub>b</sub>) of PSP, whereas 0.2 mg kg<sup>−1</sup> probenecid did not have any effect. Probenecid administered at doses of 2 and 10 mg kg<sup>−1</sup> decreased only <italic>CL</italic><sub>r</sub>. The median effective doses of probenecid for inhibiting <italic>CL</italic><sub>r</sub> and <italic>CL</italic><sub>b</sub> were 0.925 and 23.9 mg kg<sup>−1</sup>, respectively. These data suggest that a low dose of probenecid selectively inhibits urinary excretion of PSP that may be mediated by organic anion transporters, without affecting biliary excretion that may be mediated by Mrp2. Copyright © 2011 John Wiley & Sons, Ltd.</p> </abstract>