Molecular monitoring of Escherichia coli O157: H7 sterilization rate using qPCR and propidium monoazide treatment. (25th February 2013)

• Record Type: Journal Article
• Authors: Xing‐long, X.; Cong‐cong, L.; Yang, Q.; Yi‐gang, Y.; Hui, W.
• Is Part Of: Letters in applied microbiology. Volume 56:Number 5(2013:May)
• Page Start: 333
• Page End: 339
• Publication Date: 2013-02-25
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12052
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="main" xml:lang="en" id="lam12052-abs-0001"> <title>Abstract</title> <p>Propidium monoazide is a DNA‐intercalating dye. PMA‐qPCR has been reported as a novel method to detect live bacteria in complex samples. In this study, this method was used to monitor the sterilization effects of UHP, ultrasound and high PEF on <italic>Escherichia coli</italic> O157:H7. Our results showed that all three sterilization techniques are successful to kill viable <italic>E. coli</italic> O157:H7 cells under their appropriate conditions. PMA‐qPCR can effectively monitor the amount of DNA released from viable <italic>E. coli</italic> O157:H7 cells, and the results from PMA‐qPCR were highly consistent with those from plate counting after treatment with UHP, ultrasound and high PEF. The maximal Δ<italic>C</italic>_t between PMA‐qPCR and qPCR obtained in this study was 10·39 for UHP, 5·76 for ultrasound and 2·30 for high PEF. The maximal sterilization rates monitored by PMA‐qPCR were 99·92% for UHP, 99·99% for ultrasound and 100% for high PEF. Thus, PMA‐qPCR can be used to detect the sterilization effect on food and water supplies after treatment with UHP, ultrasound and high PEF.</p> </abstract>