Decreased Immunoglobulin A Concentrations in Feces, Duodenum, and Peripheral Blood Mononuclear Cells of Dogs with Inflammatory Bowel Disease. (6th December 2012)

• Record Type: Journal Article
• Authors: Maeda, S.; Ohno, K.; Uchida, K.; Nakashima, K.; Fukushima, K.; Tsukamoto, A.; Nakajima, M.; Fujino, Y.; Tsujimoto, H.
• Is Part Of: Journal of veterinary internal medicine. Volume 27:Number 1(2013:Jan./Feb.)
• Page Start: 47
• Page End: 55
• Publication Date: 2012-12-06
• Subjects: Veterinary medicine -- Periodicals; 636.0896
• DOI: 10.1111/jvim.12023
• Languages: English
• ISSNs: 0891-6640

Abstract

<abstract abstract-type="main" id="jvim12023-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jvim12023-sec-0001" sec-type="section"> <title>Background</title> <p>Although immunoglobulin A (IgA) plays a key role in regulating gut homeostasis, its role in canine inflammatory bowel disease (IBD) is unknown.</p> </sec> <sec id="jvim12023-sec-0002" sec-type="section"> <title>Hypothesis</title> <p>IgA expression may be altered in dogs with IBD, unlike that observed in healthy dogs and dogs with other gastrointestinal diseases.</p> </sec> <sec id="jvim12023-sec-0003" sec-type="section"> <title>Animals</title> <p>Thirty‐seven dogs with IBD, 10 dogs with intestinal lymphoma, and 20 healthy dogs.</p> </sec> <sec id="jvim12023-sec-0004" sec-type="section"> <title>Methods</title> <p>Prospective study. IgA and IgG concentrations in serum, feces, and duodenal samples were measured by ELISA. IgA<sup>+</sup> cells in duodenal lamina propria and IgA<sup>+</sup> CD21<sup>+</sup> peripheral blood mononuclear cells (PBMCs) were examined by immunohistochemistry and flow cytometry, respectively. Duodenal expression of the IgA‐inducing cytokine transforming growth factor β (TGF‐β), B cell activating factor (BAFF), and a proliferation‐inducing ligand (APRIL) was quantified by real‐time RT‐PCR.</p> </sec> <sec id="jvim12023-sec-0005" sec-type="section"> <title>Results</title> <p>Compared to healthy dogs, dogs with IBD had significantly decreased concentrations of IgA in fecal and duodenal samples. The number of IgA<sup>+</sup> CD21<sup>+</sup> PBMCs and IgA<sup>+</sup> cells in duodenal lamina propria was significantly lower in dogs with IBD than in healthy dogs or dogs with intestinal lymphoma. Duodenal BAFF and APRIL mRNA expression was significantly higher in IBD dogs than in the healthy controls. Duodenal TGF‐β mRNA expression was significantly lower in dogs with IBD than in healthy dogs and dogs with intestinal lymphoma.</p> </sec> <sec id="jvim12023-sec-0006" sec-type="section"> <title>Conclusions and Clinical Importance</title> <p>IBD dogs have decreased IgA concentrations in feces and duodenum and fewer IgA<sup>+</sup> PBMCs, which might contribute to development of chronic enteritis in dogs with IBD.</p> </sec> </abstract>