Drosophila splicing factor SF2 knock‐down mutant shows altered cell‐cycle in vivo. (14th January 2013)

• Record Type: Journal Article
• Authors: Dubatolova, Tatiana D.; Volkova, Elena I.; Omelyanchuk, Leonid V.
• Is Part Of: Cell biology international. Volume 37:Number 2(2013)
• Page Start: 187
• Page End: 190
• Publication Date: 2013-01-14
• Subjects: Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.10019
• Languages: English
• ISSNs: 1065-6995

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="cbin10019-sec-0001" sec-type="section"> <p>Pioneering single gene study documents pre‐mRNA processing proteins participation in the cell‐cycle regulation of multi‐cellular animals (Andersen and Tapon, 2008, J Biol Chem 283: 31256–67). Whole‐genome RNAi screen in <italic>Drosophila</italic> tissue‐culture cell lines demonstrates that 17 genes involved in RNA‐processing are required for G2/M check‐point function (Kondo and Perrimon, 2011, Sci Signal 4: rs1). In particular, the silencing of Splicing Factor 2 (SF2) increases the number of G2(M) cells. We have measured the absolute duration of cell‐cycle phases in SF2 depleted flies with the use of flow cytometry and growth parameters of GFP marked mosaic clones. For <italic>SF2</italic> mutant cells, G1 = 1.89 h, G2(M) = 7.22 h and S = 1.30 h compared with G1 = 2.25 h, G2(M) = 4.86 h and S = 1.28 h for control normal cells. Thus, G2(M) phase appears to be longer in SF2 silenced cells, supporting the evidence that this splicing protein participates in G2‐M check‐point function.</p> </sec> </abstract>