Effect of Water Extracts from Edible Myrtaceae Plants on Uptake of 2‐(n‐(7‐nitrobenz‐2‐oxa‐1, 3‐diazol‐4‐yl)amino)‐2‐deoxyglucose in TNF‐α‐Treated FL83B Mouse Hepatocytes. (25th April 2012)

• Record Type: Journal Article
• Authors: Chang, Wen‐Chang; Shen, Szu‐Chuan
• Is Part Of: Phytotherapy research. Volume 27:Number 2(2013:Feb.)
• Page Start: 236
• Page End: 243
• Publication Date: 2012-04-25
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.4681
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>This study investigated the glucose uptake activity of the water extracts from the leaves and fruit of edible Myrtaceae plants, including guava (<italic>Psidium guajava</italic> Linn.), wax apples [<italic>Syzygium samarangense</italic> (Blume) Merr. and L.M. Perry], Pu‐Tau [<italic>Syzygium jambo</italic> (L.) Alston], and Kan‐Shi Pu‐Tau (<italic>Syzygium cumini</italic> Linn.) in FL83B mouse hepatocytes. The fluorescent dye 2‐(<sc>n</sc>‐(7‐nitrobenz‐2‐oxa‐1, 3‐diazol‐4‐yl)amino)‐2‐deoxyglucose was used to estimate the uptake ability of the cells. Glucose uptake test showed that pink wax apple fruit extract (PWFE) exhibits the highest glucose uptake activity, at an increment of 21% in the insulin‐resistant FL83B mouse hepatocytes as compared with the TNF‐α‐treated control group. Vescalagin was isolated using column chromatography of PWFE. This compound, at the concentration of 6.25 µg/mL, exhibits the same glucose uptake improvement in insulin‐resistant cells as PWFE at a 100‐µg/mL dose. We postulate that vescalagin is an active component in PWFE that may alleviate the insulin resistance in mouse hepatocytes. Copyright © 2012 John Wiley &amp; Sons, Ltd.</p> </abstract>