Low‐intensity pulsed ultrasound induced enhanced adipogenesis of adipose‐derived stem cells. (22nd May 2013)

• Record Type: Journal Article
• Authors: Fu, N.; Yang, X.; Ba, K.; Fu, Y.; Wei, X.; Yue, Y.; Li, G.; Yao, Y.; Chen, J.; Cai, X.; Liang, C.; Ge, Y.; Lin, Y.
• Is Part Of: Cell proliferation. Volume 46:Number 3(2013:Jun.)
• Page Start: 312
• Page End: 319
• Publication Date: 2013-05-22
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12031
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" xml:lang="en" id="cpr12031-abs-0001"> <title>Abstract</title> <sec id="cpr12031-sec-0001" sec-type="section"> <title>Objective</title> <p>The aim of this study was to investigate effects of low‐intensity pulsed ultrasound (LIPUS) on differentiation of adipose‐derived stem cells (ASCs), <italic>in vitro</italic>.</p> </sec> <sec id="cpr12031-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes <italic>PPAR‐γ1</italic>, and <italic>APN</italic>, was examined by real‐time PCR. Immunofluorescence (IF) staining was performed to test for PPAR‐γ at the protein level.</p> </sec> <sec id="cpr12031-sec-0003" sec-type="section"> <title>Results</title> <p>Our data revealed that specific patterns of LIPUS up‐regulated levels of both PPAR‐γ1 and APN mRNA, and PPAR‐γ protein.</p> </sec> <sec id="cpr12031-sec-0004" sec-type="section"> <title>Conclusions</title> <p>In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis.</p> </sec> </abstract>