Expression of the sweetpotato R2R3‐type IbMYB1a gene induces anthocyanin accumulation in Arabidopsis. Issue 2 (6th November 2012)

• Record Type: Journal Article
• Authors: Chu, Hyosub; Jeong, Jae Cheol; Kim, Wook‐Jin; Chung, Dong Min; Jeon, Hyo Kon; Ahn, Young Ock; Kim, Sun Ha; Lee, Haeng‐Soon; Kwak, Sang‐Soo; Kim, Cha Young
• Is Part Of: Physiologia plantarum. Volume 148:Issue 2(2013:Jun.)
• Page Start: 189
• Page End: 199
• Publication Date: 2012-11-06
• Subjects: Plant physiology -- Periodicals; 571.2
• DOI: 10.1111/j.1399-3054.2012.01706.x
• Languages: English
• ISSNs: 0031-9317

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>R2R3‐type MYB transcription factors (TFs) play important roles in transcriptional regulation of anthocyanins. The R2R3‐type IbMYB1 is known to be a key regulator of anthocyanin biosynthesis in the storage roots of sweetpotato. We previously showed that transient expression of <italic>IbMYB1a</italic> led to anthocyanin pigmentation in tobacco leaves. In this article, we generated transgenic <italic>Arabidopsis</italic> plants expressing the <italic>IbMYB1a</italic> gene under the control of <italic>CaMV 35S</italic> promoter, and the sweetpotato <italic>SPO</italic> and <italic>SWPA2</italic> promoters. Overexpression of <italic>IbMYBa</italic> in transgenic <italic>Arabidopsis</italic> produced strong anthocyanin pigmentation in seedlings and generated a deep purple color in leaves, stems and seeds. Reverse transcription‐polymerase chain reaction analysis showed that <italic>IbMYB1a</italic> expression induced upregulation of several structural genes in the anthocyanin biosynthetic pathway, including <italic>4CL</italic>, <italic>CHI</italic>, <italic>F3′H</italic>, <italic>DFR</italic>, <italic>AGT</italic>, <italic>AAT</italic> and <italic>GST</italic>. Furthermore, overexpression of <italic>IbMYB1a</italic> led to enhanced expression of the <italic>AtTT8</italic> (<italic>bHLH</italic>) and <italic>PAP1</italic>/<italic>AtMYB75</italic> genes. high‐performance liquid chromatography analysis revealed that <italic>IbMYB1a</italic> expression led to the production of cyanidin as a major core molecule of anthocyanidins in <italic>Arabidopsis</italic>, as occurs in the purple leaves of sweetpotato (cv. Sinzami). This result shows that the IbMYB1a TF is sufficient to induce anthocyanin accumulation in seedlings, leaves, stems and seeds of <italic>Arabidopsis</italic> plants.</p> </abstract>