"Brainbow" imaging of developing calyx‐type presynaptic terminals in the chick ciliary ganglion. A plasmid vector containing three membrane‐tethered fl uorescent proteins, m‐CFP, m‐YFP and m‐RFP, which are flanked by loxP and its variants, were electroporated in the E2 midbrain in ovo with a plasmid containing NCre. See Yawo et al., 474‐490. (13th May 2013)