Differential Expression of Potato Defence Genes Associated with the Salicylic Acid Defence Signalling Pathway in Response to Weakly and Highly Aggressive Isolates of Verticillium dahliae. Issue 3 (12th November 2012)

• Record Type: Journal Article
• Authors: Derksen, Holly; Badawi, Mohamed; Henriquez, Maria Antonia; Yao, Zhen; El‐Bebany, Ahmed Farag; Daayf, Fouad
• Is Part Of: Journal of phytopathology. Volume 161:Issue 3(2013:Mar.)
• Page Start: 142
• Page End: 153
• Publication Date: 2012-11-12
• Subjects: Plant diseases -- Periodicals; 632
• DOI: 10.1111/jph.12038
• Languages: English
• ISSNs: 0931-1785

Abstract

<abstract abstract-type="main" id="jph12038-abs-0001"> <title>Abstract</title> <p>Verticillium wilt is a vascular disease affecting hundreds of important dicotyledonous crops worldwide. Its main causal agent in potato is <italic>Verticillium dahliae </italic>Kleb. A differential potato‐<italic>V. dahliae</italic> system consisting of two cultivars of potato (susceptible; S and moderately resistant; MR) and two <italic>V. dahliae</italic> isolates (weakly, WA and highly aggressive, HA), was used to evaluate the expression of five defence‐related genes, <italic>PAL1</italic>, <italic> PAL2</italic>, <italic> PR‐1</italic>, <italic> PR‐2</italic> and <italic>PR‐5</italic>. These genes were selected because they are in general associated with the salicylic acid defence signalling pathway. Expression levels of these genes were assessed in potato roots and leaves at 0, 4 and 21 h (hpi), and 3, 7 and 14 days postinoculation (dpi). In the roots, the expression of <italic>PAL1</italic>, <italic> PR‐1</italic> and <italic>PR‐2</italic> in the MR was higher than in the susceptible cultivar in response to inoculation with either one of the tested <italic>V. dahliae</italic> isolates. <italic>PAL2</italic> gene expression increased gradually starting at 21 hpi in the MR cultivar as opposed to the susceptible one. Higher expression of <italic>PR‐1</italic> was detected at 7 dpi in roots. In the leaves, both <italic>PAL1</italic> and <italic>PAL2</italic> genes showed higher expression in the MR cultivar relative to the susceptible one. Interestingly, the expression of <italic>PR‐2</italic> was slightly higher in the susceptible cultivar. Combined data analyses revealed that <italic>PAL1</italic>, <italic> PAL2</italic>, <italic> PR‐1</italic> and <italic>PR‐2</italic> genes are regulated at the transcriptional level in response to infection by <italic>V. dahliae</italic>. These results indicate that the salicylic acid pathway is also involved in potato defence against <italic>V. dahliae</italic> and add to the data gathered to elucidate the signalling mechanisms in this host–pathogen interaction.</p> </abstract>