OsNPR1 negatively regulates herbivore‐induced JA and ethylene signaling and plant resistance to a chewing herbivore in rice. Issue 3 (12th July 2012)

• Record Type: Journal Article
• Authors: Li, Ran; Afsheen, Sumera; Xin, Zhaojun; Han, Xiu; Lou, Yonggen
• Is Part Of: Physiologia plantarum. Volume 147:Issue 3(2013:Mar.)
• Page Start: 340
• Page End: 351
• Publication Date: 2012-07-12
• Subjects: Plant physiology -- Periodicals; 571.2
• DOI: 10.1111/j.1399-3054.2012.01666.x
• Languages: English
• ISSNs: 0031-9317

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>NPR1</italic> (a non‐expressor of pathogenesis‐related genes1) has been reported to play an important role in plant defense by regulating signaling pathways. However, little to nothing is known about its function in herbivore‐induced defense in monocot plants. Here, using suppressive substrate hybridization, we identified a <italic>NPR1</italic> gene from rice, <italic>OsNPR1</italic>, and found that its expression levels were upregulated in response to infestation by the rice striped stem borer (SSB) <italic>Chilo suppressalis</italic> and rice leaf folder (LF) <italic>Cnaphalocrocis medinalis</italic>, and to mechanical wounding and treatment with jasmonic acid (JA) and salicylic acid (SA). Moreover, mechanical wounding induced the expression of <italic>OsNPR1</italic> quickly, whereas herbivore infestation induced the gene more slowly. The antisense expression of <italic>OsNPR1</italic> (as‐<italic>npr1</italic>), which reduced the expression of the gene by 50%, increased elicited levels of JA and ethylene (ET) as well as of expression of a lipoxygenase gene <italic>OsHI‐LOX</italic> and an ACC synthase gene <italic>OsACS2</italic>. The enhanced JA and ET signaling in as‐<italic>npr1</italic> plants increased the levels of herbivore‐induced trypsin proteinase inhibitors (TrypPIs) and volatiles, and reduced the performance of SSB. Our results suggest that <italic>OsNPR1</italic> is an early responding gene in herbivore‐induced defense and that plants can use it to activate a specific and appropriate defense response against invaders by modulating signaling pathways.</p> </abstract>