Intramyocellular lipid variations in active older men: relationship with aerobic fitness. (27th December 2012)

• Record Type: Journal Article
• Authors: Rouffet, D.; Villars, C.; Fissoune, R.; Sappey‐Marinier, D.; Laville, M.; Ibarrola, D.; Sothier, M.; Monnet, M.‐F.; Ovize, M.; Bonnefoy, M.; Boesch, C.; Canet‐Soulas, E.
• Is Part Of: Acta physiologica. Volume 207:Number 3(2013:Mar.)
• Page Start: 516
• Page End: 523
• Publication Date: 2012-12-27
• Subjects: Physiology -- Periodicals; Physiology -- Research -- Periodicals; 612
• DOI: 10.1111/apha.12045
• Languages: English
• ISSNs: 1748-1708

Abstract

<abstract abstract-type="main" id="apha12045-abs-0001"> <title>Abstract</title> <sec id="apha12045-sec-0001" sec-type="section"> <title>Aims</title> <p>Intramyocellular lipid (IMCL) variations in older men are poorly explored. In young adults, IMCL can be influenced by both diet and exercise interventions; this flexibility is related to aerobic fitness. We evaluated in active older adults the influence of maximal aerobic capacity on short‐term diet and exercise‐induced variations in IMCL stores.</p> </sec> <sec id="apha12045-sec-0002" sec-type="section"> <title>Methods</title> <p>Intramyocellular lipids were measured by <sup>1</sup>H magnetic resonance spectroscopy (<sup>1</sup>H‐MRS) after a 3‐day fat depletion‐replenishment diet (IMCL<sub>FDR</sub>) and immediately after a 2‐h exercise at 50% VO<sub>2</sub> max (IMCL<sub>FDR</sub><sub>_</sub><sub>EX</sub>). To further explore diet influence, the protocol was repeated after a high‐fat diet (HF), with both pre‐ and post‐exercise measurements (IMCL<sub>HF</sub> and IMCL<sub>HF</sub><sub>_</sub><sub>EX</sub>).</p> </sec> <sec id="apha12045-sec-0003" sec-type="section"> <title>Results</title> <p>In active older men (69.8 ± 5.2 years), IMCL<sub>FDR</sub> was lowered by exercise (IMCL<sub>FDR</sub> = 3.45 ± 1.52 vs. IMCL<sub>FDR</sub><sub>_</sub><sub>EX</sub> = 2.74 ± 1.15 mmol kg<sup>−1</sup> wet weight, <italic>P</italic> < 0.05), and exercise‐induced variations were correlated to the initial store (<italic>P</italic> < 0.05, <italic>r</italic> = −0.72). IMCL<sub>FDR</sub> was linked with aerobic fitness (<italic>P</italic> < 0.05, <italic>r</italic> = 0.76), when adjusted by fat mass. IMCL<sub>HF</sub> was lower than IMCL<sub>FDR</sub> (<italic>P</italic> < 0.05), decreased after exercise (<italic>P</italic> < 0.05) and varied also as a function of initial store (<italic>P</italic> < 0.05, <italic>r</italic> = −0.89), but without link with aerobic fitness. Finally, diet‐induced IMCL store variations were positively linked to aerobic fitness (<italic>P</italic> < 0.05, <italic>r</italic> = 0.89).</p> </sec> <sec id="apha12045-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Variations of the IMCL stores in physically active older adults appear related to aerobic fitness, with similarly fast adaptation to short‐term interventions combining diet and exercise as young active adults.</p> </sec> </abstract>