Gut content analysis to study predatory efficacy of Nesidiocoris tenuis (Reuter) (Hemiptera: Miridae) by molecular methods. Issue 2 (24th October 2012)

• Record Type: Journal Article
• Authors: Itou, Masao; Watanabe, Masaya; Watanabe, Eisuke; Miura, Kazuki
• Is Part Of: Entomological science. Volume 16:Issue 2(2013:Apr.)
• Page Start: 145
• Page End: 150
• Publication Date: 2012-10-24
• Subjects: Insects -- Periodicals; Entomology -- Periodicals; 595.705
• DOI: 10.1111/j.1479-8298.2012.00552.x
• Languages: English
• ISSNs: 1343-8786

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p> <italic>Nesidiocoris tenuis</italic> is considered to make significant contributions to the control of greenhouse pests such as whiteflies, thrips, leafminers, lepidopterans, and spider mites, although there is little information based on direct observation of the predation of <italic>N. tenuis</italic> on these target pests. We developed a method to perform gut content analysis of <italic>N. tenuis</italic> based on DNA in which specific PCR primers were designed to detect the DNA of target pests. By means of gut‐content analysis, we found that the percentage of <italic>N. tenuis</italic> preying on <italic>Bemisia tabaci</italic> and <italic>Thrips palmi</italic> was approximately 40% in the field.</p> </abstract>