Accumulation of cellobiose lipids under nitrogen‐limiting conditions by two ustilaginomycetous yeasts, Pseudozyma aphidis and Pseudozyma hubeiensis. Issue 1 (11th October 2012)

• Record Type: Journal Article
• Authors: Morita, Tomotake; Fukuoka, Tokuma; Imura, Tomohiro; Kitamoto, Dai
• Is Part Of: FEMS yeast research. Volume 13:Issue 1(2013)
• Page Start: 44
• Page End: 49
• Publication Date: 2012-10-11
• Subjects: Yeast -- Periodicals; Yeasts -- Periodicals; 579.562
• DOI: 10.1111/1567-1364.12005
• Languages: English
• ISSNs: 1567-1356

Abstract

<abstract abstract-type="main" id="fyr12005-abs-0001"> <title>Abstract</title> <p>Some basidiomycetous yeast strains extracellularly produce cellobiose lipids (CLs), glycolipid biosurfactants which have strong fungicidal activity. The representative CL producer <italic>Ustilago maydis</italic> produces CLs together with the other glycolipids, mannosylerythritol lipids (MELs); the preference of the two glycolipids is affected considerably by the nitrogen source. To develop new CL producers, 12 MEL producers were cultured under the nitrogen‐limited conditions. <italic>Pseudozyma aphidis</italic> and <italic>Pseudozyma. hubeiensis</italic> were characterized as new CL producers. CL production was induced on three strains, <italic>P. aphidis, Pseudozyma graminicola</italic>, and <italic>P. hubeiensis</italic> under these conditions. The putative homologous genes of <italic>U. maydis </italic><italic>cyp1</italic>, which encodes a P450 monooxygenase, essential for CL biosynthesis, were partially amplified from their genomic DNA. The nucleotide sequences of the gene fragments from <italic>P. hubeiensis</italic> and <italic>P. aphidis</italic> shared identities with <italic>U. maydis cyp1</italic> of 99% and 78%, respectively. Furthermore, all of the deduced translation products are tightly clustered in the phylogenic tree of the monooxygenase. These results suggest that the genes involved with CL biosynthesis must be widely distributed in the basidiomycetous fungi as well as the MEL biosynthesis genes, and thus, the genus <italic>Pseudozyma</italic> has great potential as a biosurfactant producer.</p> </abstract>