Fuel ethanol production from Eucalyptus globulus wood by autocatalized organosolv pretreatment ethanol–water and SSF. Issue 1 (7th August 2012)

• Record Type: Journal Article
• Authors: Yáñez‐S, Mauricio; Rojas, Jonathan; Castro, Jean; Ragauskas, Arthur; Baeza, Jaime; Freer, Juanita
• Is Part Of: Journal of chemical technology & biotechnology. Volume 88:Issue 1(2013:Jan.)
• Page Start: 39
• Page End: 48
• Publication Date: 2012-08-07
• Subjects: Biotechnology -- Periodicals; Chemistry, Technical -- Periodicals; Chemical engineering -- Periodicals; Industries -- Environmental aspects -- Periodicals; 660
• DOI: 10.1002/jctb.3895
• Languages: English
• ISSNs: 0268-2575

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jctb3895-sec-0100" sec-type="section"> <title>BACKGROUND</title> <p> <bold>Lignocellulosic biomass (LCB) offers the only sustainable alternative to the use of fossil fuels as oil by employing its main components (cellulose, hemicellulose and lignin) as a carbon source for the production of biofuels, energy and value‐added chemicals. The aim of the present study was to investigate ethanol production from organosolv pretreated <italic>Eucalyptus globulus</italic> wood, carrying out the simultaneous sacharification and fermentation (SSF) process at a substrate loading of 10 and 15% (w/v) and using concentrations of 6 and 12 g L<sup>−1</sup> of the thermally acclimatized <italic>S. cerevisiae</italic> IR2T9‐a strain. These SSF experiments were also evaluated by increasing the enzyme loading in the reaction medium. Finally, a comparison was made between separate hydrolysis and fermentation (SHF) and SSF processes. With the information obtained and from published information, a general mass balance was developed.</bold> </p> </sec> <sec id="jctb3895-sec-0101" sec-type="section"> <title>RESULTS</title> <p> <bold>The highest ethanol concentration (∼42 g L<sup>−1</sup>) in the fermentation broth was obtained at a substrate consistency of 15% (w/v), enzyme loading of 20 FPU cellulase 40 UI β‐glucosidase g<sup>−1</sup> of pretreated material and using both <italic>S. cerevisiae</italic> strain IR2‐9a concentrations (6 and 12 g L<sup>−1</sup>).</bold> </p> </sec> <sec id="jctb3895-sec-0102" sec-type="section"> <title>CONCLUSION</title> <p> <bold>The results of enzymatic hydrolysis (EH) show that increasing substrate content from 10 to 15% (w/v) decreases the conversion efficiency of cellulose to glucose. Furthermore, the mass balances of the process indicate that the SSF process is a better alternative than the SHF configuration, because larger amounts of ethanol can be obtained. Copyright © 2012 Society of Chemical Industry</bold> </p> </sec> </abstract>