Polymorphisms in the promoter region of the adiponectin (ADIPOQ) gene are presumably associated with transcription level and carcass traits in pigs. (20th July 2012)

• Record Type: Journal Article
• Authors: Cieslak, J.; Flisikowska, T.; Schnieke, A.; Kind, A.; Szydlowski, M.; Switonski, M.; Flisikowski, K.
• Is Part Of: Animal genetics. Volume 44:Number 3(2013:Jun.)
• Page Start: 340
• Page End: 343
• Publication Date: 2012-07-20
• Subjects: Animal genetics -- Periodicals; 572.8
• DOI: 10.1111/j.1365-2052.2012.02397.x
• Languages: English
• ISSNs: 0268-9146

Abstract

<abstract abstract-type="main" id="age2397-abs-0001"> <title>Summary</title> <p>The main goal of this study was to screen for polymorphisms in the porcine adiponectin (<italic>ADIPOQ</italic>) gene promoter, analyse their influence on transcription and identify any association with production traits in pigs. A 1018‐bp region of the <italic>ADIPOQ</italic> gene promoter was analysed in 113 pigs, and seven novel polymorphisms found. Luciferase assays were performed in HEK293 (human embryonic kidney) cells and primary porcine adipose mesenchymal stem cells (pADMSCs) to investigate their affect on promoter activity. A 16‐bp indel (c.−106_−91delGCCAGGGGTGTGAGCC) was found to influence promoter strength <italic>in vitro</italic>. In the HEK293 cell line, the Del/Del genotype showed greater luciferase activity than did the Ins/Ins genotype (<italic>P </italic>&lt;<italic> </italic>0.01). In pADMSCs, the insertion genotype of the <italic>ADIPOQ</italic> promoter showed greater luciferase activity than did the deletion genotype (<italic>P </italic>&lt;<italic> </italic>0.01). An association study performed for two novel polymorphisms, c.−67G&gt;A and the 16‐bp indel, showed significant correlation with loin measurements in Polish Landrace (<italic>P </italic>&lt;<italic> </italic>0.05) and synthetic line 990 (<italic>P </italic>&lt;<italic> </italic>0.01) pigs.</p> </abstract>