Antimicrobial resistance in faecal enterococci and Escherichia coli isolates recovered from Iberian wolf. (8th February 2013)

• Record Type: Journal Article
• Authors: Gonçalves, A.; Igrejas, G.; Radhouani, H.; Correia, S.; Pacheco, R.; Santos, T.; Monteiro, R.; Guerra, A.; Petrucci‐Fonseca, F.; Brito, F.; Torres, C.; Poeta, P.
• Is Part Of: Letters in applied microbiology. Volume 56:Number 4(2013:Apr.)
• Page Start: 268
• Page End: 274
• Publication Date: 2013-02-08
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12044
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="main" xml:lang="en" id="lam12044-abs-0001"> <title>Abstract</title> <p>The aim of this study was to report the antimicrobial resistance, the molecular mechanisms associated and the detection of virulence determinants within faecal <italic>Enterococcus</italic> spp. and <italic>Escherichia coli</italic> isolates of Iberian wolf. Enterococci (<italic>n </italic>=<italic> </italic>227) and <italic>E. coli</italic> (<italic>n </italic>=<italic> </italic>195) isolates were obtained from faecal samples of Iberian wolf (<italic>Canis lupus signatus</italic>). High rates of resistance were detected for tetracycline and erythromycin among the enterococci isolates, and most of resistant isolates harboured the <italic>tet</italic>(M) and/or <italic>tet</italic>(L) and <italic>erm</italic>(B) genes, respectively. The <italic>bla</italic><sub>TEM, </sub><italic>tet</italic>(A) and/or <italic>tet</italic>(B), and <italic>aadA</italic> or <italic>strA</italic>‐<italic>strB</italic> genes were detected among most ampicillin‐, tetracycline‐ or streptomycin‐resistant <italic>E. coli</italic> isolates, respectively. <italic>E. coli</italic> isolates were ascribed to phylogroups A (<italic>n </italic>=<italic> </italic>56), B1 (91), B2 (13) and D (35). The occurrence of resistant enterococci and <italic>E. coli</italic> isolates in the faecal flora of Iberian wolf, including the presence of resistant genes in integrons, and virulence determinants was showed in this study. Iberian wolf might act as reservoir of certain resistance genes that could be spread throughout the environment.</p> </abstract>