Transcriptional repression by glycerol of genes involved in the assimilation of n‐alkanes and fatty acids in yeast Yarrowia lipolytica. Issue 2 (14th January 2013)

• Record Type: Journal Article
• Authors: Mori, Katsuki; Iwama, Ryo; Kobayashi, Satoshi; Horiuchi, Hiroyuki; Fukuda, Ryouichi; Ohta, Akinori
• Is Part Of: FEMS yeast research. Volume 13:Issue 2(2013)
• Page Start: 233
• Page End: 240
• Publication Date: 2013-01-14
• Subjects: Yeast -- Periodicals; Yeasts -- Periodicals; 579.562
• DOI: 10.1111/1567-1364.12025
• Languages: English
• ISSNs: 1567-1356

Abstract

<abstract abstract-type="main" id="fyr12025-abs-0001"> <title>Abstract</title> <p>The yeast <italic>Yarrowia lipolytica</italic> assimilates <italic>n</italic>‐alkanes or fatty acids as carbon sources. Transcriptional activation by <italic>n</italic>‐alkanes of <italic>ALK1</italic> encoding a cytochrome P450 responsible for the terminal hydroxylation has been well studied so far, but its regulation by other carbon sources is poorly understood. Here, we analyzed the transcriptional regulation of <italic>ALK1</italic> by glycerol. Glycerol is a preferable carbon source compared to glucose for <italic>Y. lipolytica</italic>. The <italic>n</italic>‐decane‐induced transcript levels of <italic>ALK1</italic> as well as the reporter gene under the control of <italic>ALK1</italic> promoter were significantly decreased in the simultaneous presence of glycerol, but not of glucose. Similarly, the expression of <italic>PAT1</italic> encoding acetoacetyl‐CoA thiolase involved in β‐oxidation was induced by <italic>n</italic>‐decane or oleic acid, but its transcript level was decreased when glycerol was supplemented. These results indicate that glycerol represses the transcription of the genes involved in the metabolism of hydrophobic carbon sources in <italic>Y. lipolytica</italic>. Repression of <italic>ALK1</italic> transcription by glycerol was not observed in the deletion mutant of <italic>GUT1</italic> encoding glycerol kinase, implying that the phosphorylation of glycerol is required for the glycerol repression.</p> </abstract>