Novel neuroprotective action of prothymosin alpha‐derived peptide against retinal and brain ischemic damages. (28th January 2013)

• Record Type: Journal Article
• Authors: Halder, Sebok Kumar; Matsunaga, Hayato; Yamaguchi, Haruka; Ueda, Hiroshi
• Is Part Of: Journal of neurochemistry. Volume 125:Number 5(2013:Jun.)
• Page Start: 713
• Page End: 723
• Publication Date: 2013-01-28
• Subjects: Neurochemistry -- Periodicals; 616.8042
• DOI: 10.1111/jnc.12132
• Languages: English
• ISSNs: 0022-3042

Abstract

<abstract abstract-type="main" id="jnc12132-abs-0001"> <title>Abstract</title> <p>Prothymosin alpha (ProTα), a nuclear protein, is implicated in the inhibition of ischemia‐induced necrosis as well as apoptosis in the brain and retina. Although ProTα has multiple biological functions through distinct regions in its sequence, it has remained which region is involved in this neuroprotection. This study reported that the active core peptide sequence P<sub>30</sub> (amino acids 49–78) of ProTα exerts its full survival effect in cultured cortical neurons against ischemic stress. Our <italic>in vivo</italic> study revealed that intravitreous administration of P<sub>30</sub> at 24 h after retinal ischemia significantly blocks the ischemia‐induced functional damages of retina at day 7. In addition, P<sub>30</sub> completely rescued the retinal ischemia‐induced ganglion cell damages at day 7 after the ischemic stress, along with partial blockade of the loss of bipolar, amacrine, and photoreceptor cells. On the other hand, intracerebroventricular (3 nmol) or systemic (1 mg/kg; i.v.) injection of P<sub>30</sub> at 1 h after cerebral ischemia (1 h tMCAO) significantly blocked the ischemia‐induced brain damages and disruption of blood vessels. Systemic P<sub>30</sub> delivery (1 mg/kg; i.v.) also significantly ameliorated the ischemic brain caused by photochemically induced thrombosis. Taken together, this study confers a precise demonstration about the novel protective activity of ProTα‐derived small peptide P<sub>30</sub> against the ischemic damages <italic>in vitro</italic> and <italic>in vivo</italic>.</p> </abstract>