Genotyping of Rhodopirellula baltica organisms using multispacer sequence typing. (18th April 2013)

• Record Type: Journal Article
• Authors: Cayrou, Caroline; Terra, Anthony; Drancourt, Michel
• Is Part Of: Marine ecology. Volume 34:Number 2(2013:Jun.)
• Page Start: 251
• Page End: 254
• Publication Date: 2013-04-18
• Subjects: Marine ecology -- Periodicals; 577.705
• DOI: 10.1111/maec.12015
• Languages: English
• ISSNs: 0173-9565

Abstract

<abstract abstract-type="main" xml:lang="en" id="maec12015-abs-0001"> <title>Abstract</title> <p> <italic>Rhodopirellula baltica</italic> is a ubiquitous Planctomycetes organism isolated from marine environments worldwide. Multilocus sequence analysis (MLSA) has been the only method used to assess the diversity of <italic>R. baltica</italic> organisms. Here, we developed multispacer sequence typing (MST) genotyping and applied it to four <italic>R. baltica</italic> isolates from the French Mediterranean Sea and <italic>R. baltica</italic> DSM10527^T type strain. While MLSA yielded interpretable sequences for two of nine genes in three <italic>R. baltica</italic> isolates, MST based on sequencing two intergenic spacers yielded an unambiguous, strain‐specific profile for each one of the four <italic>R. baltica</italic> isolates under investigation. We propose that MST could be used as an alternative <italic>R. baltica</italic> genotyping method to assess the diversity of these Planctomycetes organisms.</p> </abstract>