Oral immunization of mice with Saccharomyces cerevisiae expressing a neutralizing epitope of ApxIIA exotoxin from Actinobacillus pleuropneumoniae induces systemic and mucosal immune responses. (16th June 2013)

• Record Type: Journal Article
• Authors: Shin, Min‐Kyoung; Lee, Won‐Jung; Jung, Myung Hwan; Cha, Seung‐Bin; Shin, Seung‐Won; Yoo, Anna; Kim, Dae‐Hyuk; Yoo, Han Sang
• Is Part Of: Microbiology and immunology. Volume 57:Number 6(2013:Jun.)
• Page Start: 417
• Page End: 425
• Publication Date: 2013-06-16
• Subjects: Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12053
• Languages: English
• ISSNs: 0385-5600

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="mim12053-sec-0001" sec-type="section"> <p>An oral delivery system based on ApxIIA#5‐expressed on <italic>Saccharomyces cerevisiae</italic> was studied for its potential to induce immune responses in mice. Murine bone marrow‐derived dendritic cells (DCs) stimulated <italic>in vitro</italic> with ApxIIA#5‐expressed on <italic>S. cerevisiae</italic> upregulated the expression of maturation and activation markers, leading to production of tumor necrosis factor‐α, interleukin (IL)‐1β, IL‐12p70 and IL‐10. Presentation of these activated DCs to cluster of differentiation CD4+ T cells collected from mice that had been orally immunized with the ApxIIA#5‐expressed on <italic>S. cerevisiae</italic> elicited specific T‐cell proliferation. In addition, the orally immunized mice had stronger antigen‐specific serum IgG and IgA antibody responses and larger numbers of antigen‐specific IgG and IgA antibody‐secreting cells in their spleens, Peyer's patches and lamina propria than did those immunized with vector‐only <italic>S. cerevisiae</italic> or those not immunized. Furthermore, oral immunization induced T helper 1‐type immune responses mediated via increased serum concentrations of IgG2a and an increase predominantly of IFN‐γ‐producing cells in their spleens and lamina propria. Our findings suggest that surface‐displayed ApxIIA#5‐expressed on <italic>S. cerevisiae</italic> may be a promising candidate for an oral vaccine delivery system for eliciting systemic and mucosal immunity.</p> </sec> </abstract>