The combination of DHEA, histamine, and insulin increases adipogenic differentiation and enhances tissue transplantation outcome in mice. (19th June 2013)

• Record Type: Journal Article
• Authors: Park, Yoorim; Jung, Min Kyung; Yoon, Sun Young; Lee, Ha‐Reum; Hur, Dae Young; Kim, Daejin; Yang, Yoolhee; Kim, Tae Sung; Kim, Seonghan; Yoon, Suk Ran; Park, Hyun Jeong; Bang, Sa Ik; Cho, Dae Ho
• Is Part Of: Biotechnology and applied biochemistry. Volume 60:Number 3(2013:May/Jun.)
• Page Start: 356
• Page End: 364
• Publication Date: 2013-06-19
• Subjects: Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1100
• Languages: English
• ISSNs: 0885-4513

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Adipose stem cells (ASCs) are pluripotent cells that can generate pure fat tissue for regeneration. Differentiated adipose cells have been generated by a common inducer cocktail composed of dexamethasone, insulin, and isobutylmethylxanthine (DIM). The major drawbacks of adipose cells are their tendency to float on the culture media and their cost. To overcome some of these disadvantages, a new inducer cocktail that includes insulin, dehydroepiandrosterone, and histamine (D<sub>H</sub>IH) was tested. As a result, lipid accumulation was elevated more than twofold with D<sub>H</sub>IH than with DIM. Cell adhesion and viability, which are important factors for stable differentiation, were increased with D<sub>H</sub>IH and were proven through measurement of mRNA expression levels of adhesion marker genes, N‐cadherin and vascular cell adhesion molecule, as well as through an alamar blue assay. The expression of adipogenesis‐related genes, adiponectin, and glucose transporter type 4 lasted for a long time. To improve the efficiency of grafting, cell adhesion and neovascularization need to be increased. Neovascularization was observed around the transplanted adipose cells, which showed a higher number of vessel formation in D<sub>H</sub>IH than in DIM. The above results suggest that D<sub>H</sub>IH can produce pure differentiated adipose cells effectively and enhance their adhesion onto the target location when these differentiated adipose cells were applied as a clinical resource.</p> </abstract>