A DNA microarray for the versatile diagnosis of infectious diarrhea. Issue 7 (12th June 2013)

• Record Type: Journal Article
• Authors: Donatin, Emilie; Buffet, Sylvain; Leroy, Quentin; Raoult, Didier; Drancourt, Michel
• Is Part Of: Apmis. Volume 121:Issue 7(2013:Jul.)
• Page Start: 634
• Page End: 642
• Publication Date: 2013-06-12
• Subjects: Pathology -- Periodicals; Microbiology -- Periodicals; Immunology -- Periodicals; 572
• DOI: 10.1111/apm.12081
• Languages: English
• ISSNs: 0903-4641

Abstract

<abstract abstract-type="main" id="apm12081-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Several bacteria, viruses, and parasites cause diarrhea as coinfecting pathogens. We designed a DNA microarray comprising 60‐bp probes spotted 194 times for the multiplex detection of 33 enteropathogenic bacteria and seven enteropathogenic viruses, and the archaeon <italic>Methanobrevibacter smithii</italic> was used as an internal positive control. Nine pathogen‐free stool specimens were used as negative controls. One of these control specimens was further spiked with <italic>Salmonella enterica</italic> as a positive control. The microarray was then tested with 40 pathological stool specimens, comprising <italic>S. enterica</italic> (n = 30), <italic>Campylobacter jejuni</italic> (n = 4), pathogenic <italic>Escherichia coli</italic> (n = 2), and adenovirus (n = 4). <italic>M. smithii</italic> was detected in 47/49 (95.9%) specimens, no pathogen was detected in negative controls and <italic>S. enterica</italic> was identified in the <italic>S. enterica‐</italic>spiked positive control. The overall specificity was 100% and the overall sensitivity was 97.5% because one <italic>S. enterica</italic> sample was missed by the microarray. The multiplexed detection of <italic>C. jejuni</italic> spiked into an adenovirus‐positive stool sample gave positive results, with fluorescence values of 14.3 and 9.1, respectively. These data indicate that using the protocol developed in this article, the DNA array allows for the multiplexed detection of some enteropathogens in stool samples.</p> </abstract>