MicroRNAs as tumour suppressors in canine and human melanoma cells and as a prognostic factor in canine melanomas. (3rd May 2013)

• Record Type: Journal Article
• Authors: Noguchi, S.; Mori, T.; Hoshino, Y.; Yamada, N.; Maruo, K.; Akao, Y.
• Is Part Of: Veterinary and comparative oncology. Volume 11:Number 2(2013:Jun.)
• Page Start: 113
• Page End: 123
• Publication Date: 2013-05-03
• Subjects: Veterinary oncology -- Periodicals; Neoplasms -- veterinary -- Periodicals; 636.0896994005
• DOI: 10.1002/vco.306
• Languages: English
• ISSNs: 1476-5810

Abstract

<abstract abstract-type="main" id="vco306-abs-0001"> <title>Abstract</title> <p>Malignant melanoma (MM) is one of the most aggressive cancers in dogs and in humans. However, the molecular mechanisms of its development and progression remain unclear. Presently, we examined the expression profile of microRNAs (miRs) in canine oral MM tissues and paired normal oral mucosa tissues by using the microRNA‐microarray assay and quantitative RT‐PCR. Importantly, a decreased expression of miR‐203 was significantly associated with a shorter survival time. Also, miR‐203 and ‐205 were markedly down‐regulated in canine and human MM cell lines tested. Furthermore, the ectopic expression of miR‐205 had a significant inhibitory effect on the cell growth of canine and human melanoma cells tested by targeting <italic>erbb3</italic>. Our data suggest that miR‐203 is a new prognostic factor in canine oral MMs and that miR‐205 functions as a tumour suppressor by targeting <italic>erbb3</italic> in both canine and human MM cells.</p> </abstract>