Histology of saphenous veins after treatment with the ClariVein® device – an ex‐vivo experiment. (26th February 2013)

• Record Type: Journal Article
• Authors: Kendler, Michael; Averbeck, Marco; Simon, Jan C.; Ziemer, Mirjana
• Is Part Of: Journal der Deutschen Dermatologischen Gesellschaft. Volume 11:Number 4(2013)
• Page Start: 348
• Page End: 352
• Publication Date: 2013-02-26
• Subjects: Skin -- Diseases -- Periodicals; Dermatology -- Periodicals; 616.5005
• DOI: 10.1111/ddg.12022
• Languages: English
• ISSNs: 1610-0379

Abstract

<abstract abstract-type="main"> <title>Summary</title> <sec id="ddg12022-sec-0010" sec-type="section"> <title>Background</title> <p>Endovenous treatment modalities are used increasingly to treat varicose veins. The ClariVein® catheter is a new endoluminal mechanico‐chemical obliteration technique which can be used without tumescent anesthesia. It is still unclear what changes the mechanical tip of the catheter has on the walls of the vein.</p> </sec> <sec id="ddg12022-sec-0020" sec-type="section"> <title>Patients and methods</title> <p>Five great saphenous vein specimens were obtained atraumatically by crossectomy. Then the veins were treated ex vivo with the ClariVein® catheter without sclerotherapy. The activated catheter rotating tip (3 500 U/min) was steadily withdrawn at 1–2 mm per second. Subsequently, histological and immunohistochemical investigations of treated (cv) and untreated specimens (plain) were performed. A 4‐point score was calculated to compare the results.</p> </sec> <sec id="ddg12022-sec-0030" sec-type="section"> <title>Results</title> <p>The mechanical part of the catheter caused a subtle incomplete destruction of the endothelium (endothelium cv: 2.2 vs. plain: 1, p = 0.04). Changes in the media or adventitia were not seen. Immunohistochemical presentation of the endothelium of the intima was demonstrated with antibodies against CD31 (cv: 3.4 vs. plain: 2.8), CD34 (cv: 3.8 vs. plain: 3.2) and factor VIII (cv: 2.2 vs. plain: 1, p = 0, 004).</p> </sec> <sec id="ddg12022-sec-0040" sec-type="section"> <title>Conclusions</title> <p>The mechanical part of the ClariVein® catheter caused a subtle incomplete destruction of endothelium, which was confirmed histologically and immunohistochemically. The reduced expression of factor VIII in the treated vein could be caused by the release of preformed factor VIII granules due to the minimal mechanical irritation.</p> </sec> </abstract>