Periodontal tissue engineering with stem cells from the periodontal ligament of human retained deciduous teeth. (8th August 2012)

• Record Type: Journal Article
• Authors: Ji, K.; Liu, Y.; Lu, W.; Yang, F.; Yu, J.; Wang, X.; Ma, Q.; Yang, Z.; Wen, L.; Xuan, K.
• Is Part Of: Journal of periodontal research. Volume 48:Number 1(2013:Feb.)
• Page Start: 105
• Page End: 116
• Publication Date: 2012-08-08
• Subjects: Periodontics -- Periodicals; 617.632
• DOI: 10.1111/j.1600-0765.2012.01509.x
• Languages: English
• ISSNs: 0022-3484

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Background and Objective: </bold> Periodontal ligament stem cells from human permanent teeth (PePDLSCs) have been investigated extensively in periodontal tissue engineering and regeneration. However, little knowledge is available on the periodontal ligament stem cells from human retained deciduous teeth (DePDLSCs). This study evaluated the potential of DePDLSCs in periodontal tissue regeneration.</p> <p> <bold>Material and Methods: </bold> DePDLSCs were isolated and purified by limited dilution. The characteristics of DePDLSCs were evaluated and compared with PePDLSCs both <italic>in vitro</italic> and <italic>in vivo</italic>.</p> <p> <bold>Results: </bold> DePDLSCs presented a higher proliferation rate and colony‐forming capacity than PePDLSCs <italic>in vitro</italic>. During the osteogenic induction, alkaline phosphatase (ALP) activity, mineralized matrix formation and expression of mineralization‐related genes, including runt‐related transcription factor 2 (<italic>RUNX2</italic>), <italic>ALP</italic>, collagen type I (<italic>COLI</italic>) and osteocalcin (<italic>OCN</italic>) were significantly enhanced in DePDLSCs compared with PePDLSCs. Furthermore, DePDLSC cell sheets showed a stronger synthesis of collagen type I in the extracellular matrix than did PePDLSC cell sheets. After <italic>in vivo</italic> transplantation, DePDLSC cell sheets recombined with human dentin blocks were able to generate new cementum/periodontal ligament‐like tissues.</p> <p> <bold>Conclusion: </bold> Our findings suggest that DePDLSCs can be used as a promising candidate for periodontal tissue engineering.</p> </abstract>