Long non‐coding RNA HOTAIR is an independent prognostic marker for nasopharyngeal carcinoma progression and survival. Issue 4 (30th January 2013)

• Record Type: Journal Article
• Authors: Nie, Yan; Liu, Xiang; Qu, Shaohua; Song, Erwei; Zou, Hua; Gong, Chang
• Is Part Of: Cancer science. Volume 104:Issue 4(2013:Apr.)
• Page Start: 458
• Page End: 464
• Publication Date: 2013-01-30
• Subjects: Cancer -- Periodicals; Neoplasms -- Periodicals; Research -- Periodicals; Electronic journals; 616.994005
• DOI: 10.1111/cas.12092
• Languages: English
• ISSNs: 1347-9032

Abstract

<abstract abstract-type="main" id="cas12092-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Identification of new nasopharyngeal carcinoma (NPC) biomarkers is of great clinical value for the diagnosis and treatment of NPC. <italic>HOTAIR</italic>, a cancer‐related long non‐coding RNA, was tested and its prognostic value for NPC was evaluated. As determined using <italic>in situ</italic> hybridization (ISH), 91 of 160 (56.87%) paraffin‐embedded NPC biopsies showed high expression levels of <italic>HOTAIR</italic> (staining index score ≥ 6). <italic>HOTAIR</italic> was upregulated in tumors with a large size (<italic>P </italic>=<italic> </italic>0.021), more advanced clinical stage (<italic>P </italic>=<italic> </italic>0.012) and increased lymph node tumor burden (<italic>P </italic>=<italic> </italic>0.005). Quantified using real‐time PCR, <italic> HOTAIR</italic> expression levels in fresh tissue and paraffin‐embedded samples were 5.2 ~ 48.4‐fold higher compared with non‐cancer tissue samples. Moreover, <italic>HOTAIR</italic> expression levels increased with clinical stage progression, which was consistent with ISH findings in the paraffin‐embedded tissue. Most importantly, NPC patients with higher <italic>HOTAIR</italic> levels had a poor prognosis for overall survival using univariate and multivariate analysis. In addition, <italic>HOTAIR</italic> mediated the migration, invasion and proliferation of NPC cells <italic>in vitro</italic>. <italic>HOTAIR</italic> is a potential biomarker for the prognosis of NPC, and dysregulation of <italic>HOTAIR</italic> might play an important role in NPC progression.</p> </abstract>