Association between Genetic Polymorphisms of NOD 1 and Helicobacter pylori‐Induced Gastric Mucosal Inflammation in Healthy Korean Population. Issue 2 (8th November 2012)

• Record Type: Journal Article
• Authors: Kim, Eun Jung; Lee, Jeong Rok; Chung, Woo Chul; Jung, Sung Hoon; Sung, Hae Jung; Lee, Yang Woon; Oh, You Suk; Kim, Sang Bae; Paik, Chang Nyol; Lee, Kang‐Moon; Noh, Seung June
• Is Part Of: Helicobacter. Volume 18:Issue 2(2013:Apr.)
• Page Start: 143
• Page End: 150
• Publication Date: 2012-11-08
• Subjects: Helicobacter -- Periodicals; Helicobacter infections -- Periodicals; Stomach -- Diseases -- Periodicals; 616.3301405
• DOI: 10.1111/hel.12020
• Languages: English
• ISSNs: 1083-4389

Abstract

<abstract abstract-type="main" id="hel12020-abs-0001"> <title>Abstract</title> <sec id="hel12020-sec-0001" sec-type="section"> <title>Background</title> <p>Gastric cancer is supposed to be a result of inflammation induced by <italic>Helicobacter pylori</italic> (<italic>H. pylori</italic>) infection. Nucleotide‐binding oligomerization domain 1 (NOD 1) is required for the innate immune response to <italic>H. pylori</italic>. We aim to investigate whether single nucleotide polymorphism (SNP) in NOD 1 gene is associated with <italic>H. pylori</italic>‐induced gastric mucosal inflammation in a healthy Korean population.</p> </sec> <sec id="hel12020-sec-0002" sec-type="section"> <title>Methods</title> <p>The study was conducted on 412 adults who visited two different healthcare centers for health examinations. The G796A (E266K) NOD 1 SNP was detected by using polymerase chain reaction/restriction fragment length polymorphism. A gastritis score was calculated by the summed values of the grade and the activity of gastritis scored according to the updated Sydney system. The expression of IL‐8 and COX‐2 mRNA was assessed by quantitative reverse transcription polymerase chain reaction. In the group with <italic>H. pylori</italic> infection, the complete screening of the genes comprising the cag PAI was performed.</p> </sec> <sec id="hel12020-sec-0003" sec-type="section"> <title>Results</title> <p>The genotype frequencies were 26.7% (AA type), 58.3% (GA), and 15.0% (GG). In <italic>H. pylori</italic>‐positive patients, gastritis score of the AA genotype was significantly higher than those of the others (<italic>p </italic>=<italic> </italic>.04). Also, the IL‐8 and COX‐2 mRNA levels increased in the AA genotype. In the group with <italic>H. pylori</italic> infection, 31.9% were found to carry the complete cag PAI. When the subjects were infected with intact cag PAI, the IL‐8 and COX‐2 mRNA levels were significantly high in AA genotype.</p> </sec> <sec id="hel12020-sec-0004" sec-type="section"> <title>Conclusion</title> <p>G796A (E266K) NOD 1 polymorphism is closely correlated with <italic>H. pylori</italic>‐associated gastric mucosal inflammation in the Korean population.</p> </sec> </abstract>