Heat Shock Protein 90 of Bonamia ostreae: Characterization and Possible Correlation with Infection of the Flat Oyster, Ostrea edulis. (12th March 2013)

• Record Type: Journal Article
• Authors: Prado‐Alvarez, Maria; Chollet, Bruno; Couraleau, Yann; Morga, Benjamin; Arzul, Isabelle
• Is Part Of: Journal of eukaryotic microbiology. Volume 60:Number 3(2013:May/Jun.)
• Page Start: 257
• Page End: 266
• Publication Date: 2013-03-12
• Subjects: Protista -- Periodicals; Eukaryotic cells -- Periodicals; Microbiology -- Periodicals; 579.05
• DOI: 10.1111/jeu.12031
• Languages: English
• ISSNs: 1066-5234

Abstract

<abstract abstract-type="main" id="jeu12031-abs-0001"> <title>Abstract</title> <p>In this study, we described the cytosolic HSP90 of <italic>Bonamia ostreae</italic>, an intracellular parasite of <italic>Ostrea edulis</italic> hemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of <italic>B. ostreae</italic>‐infected hemocytes. <italic>HSP90</italic> amplification was corroborated in infected oysters and <italic>B. ostreae</italic> purified cells. The functionality of the HSP90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in hemocyte invasion. Our results inform the molecular basis that governs <italic>B. ostreae–O. edulis</italic> interactions.</p> </abstract>