Flow cytometry thresholds of myeloperoxidase detection to discriminate between acute lymphoblastic or myeloblastic leukaemia. (25th February 2013)

• Record Type: Journal Article
• Authors: Guy, Julien; Antony‐Debré, Iléana; Benayoun, Emmanuel; Arnoux, Isabelle; Fossat, Chantal; Le, Magali; Raimbault, Anna; Imbert, Michèle; Maynadié, Marc; Lacombe, Francis; Béné, Marie C; Wagner‐Ballon, Orianne
• Is Part Of: British journal of haematology. Volume 161:Number 4(2013:May)
• Page Start: 551
• Page End: 555
• Publication Date: 2013-02-25
• Subjects: Hematology -- Periodicals; Blood -- Diseases -- Periodicals; 616.15
• DOI: 10.1111/bjh.12277
• Languages: English
• ISSNs: 0007-1048

Abstract

<abstract abstract-type="main" xml:lang="en" id="bjh12277-abs-0001"> <title>Summary</title> <p>The World Health Organization 2008 Classification emphasizes myeloperoxidase (MPO) detection as sufficient for assigning a blast population to the myeloid lineage. Published MPO positivity thresholds are 10% for flow cytometry (FCM) but 3% for cytochemistry. Here we re‐evaluated the FCM‐MPO threshold by comparing retrospectively 128 acute lymphoblastic leukaemias and 75 acute myeloid leukaemias without maturation, all assessed by benzidine‐based cytochemistry. A 13% threshold was found to be relevant using an isotype control as background‐reference (sensitivity 95·1%, specificity 91·7%). Residual normal lymphocytes proved to be an advantageous alternative reference, a threshold of 28% yielding improved 97·4% sensitivity and 96·1% specificity.</p> </abstract>